Western Blot (WB)
(Anti-PMVK antibody, MBS175363, Western blottingWB: Rat Cardiac Muscle Tissue Lysate )
Rabbit PMVK Polyclonal Antibody | anti-PMVK antibody
Anti-PMVK antibody
Gene Names
PMVK; PMK; PMKA; PMKASE; HUMPMKI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
PMVK; Polyclonal Antibody; Anti-PMVK antibody; Phosphomevalonate kinase(PMKase/hPMK); phosphomevalonate kinase; hPMK antibody; HUMPMKI antibody; OTTHUMP00000035546 antibody; Phosphomevalonate kinase antibody; PMK antibody; PMK PEN antibody; PMKA antibody; PMKase antibody; PMKI antibody; PMKPEN antibody; PMVK antibody; PMVK_HUMAN antibody; anti-PMVK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
192
Applicable Applications for anti-PMVK antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PMVK (135-151aa EQSRQQRGWVFTPGVDD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-PMVK antibody, MBS175363, Western blottingWB: Rat Cardiac Muscle Tissue Lysate )
Western Blot (WB)
(Anti-PMVK antibody, MBS175363, Western blottingWB: Rat Cardiac Muscle Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-PMVK antibody, MBS175363, IHC(P)IHC(P): Rat Skeletal Muscle Tissue )
Immunohistochemistry (IHC)
(Anti-PMVK antibody, MBS175363, IHC(P)IHC(P): Rat Skeletal Muscle Tissue )
Related Product Information for anti-PMVK antibody
Description: Rabbit IgG polyclonal antibody for Phosphomevalonate kinase(PMVK) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: PMVK(phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence(PTS-1), Ser-Arg-Leu, at the C terminus of the protein.
Background: PMVK(phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence(PTS-1), Ser-Arg-Leu, at the C terminus of the protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,995 Da
NCBI Official Full Name
Phosphomevalonate kinase
NCBI Official Synonym Full Names
phosphomevalonate kinase
NCBI Official Symbol
PMVK
NCBI Official Synonym Symbols
PMK; PMKA; PMKASE; HUMPMKI
NCBI Protein Information
phosphomevalonate kinase; hPMK
UniProt Protein Name
Phosphomevalonate kinase
UniProt Gene Name
PMVK
UniProt Synonym Gene Names
PMKI; PMKase; hPMK
UniProt Entry Name
PMVK_HUMAN
NCBI Description
This gene encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. [provided by RefSeq, Sep 2011]
Uniprot Description
PMVK: Induced by sterol. Monomer.
Protein type: Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.7.4.2; Kinase, other
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: membrane; peroxisome; cytosol
Molecular Function: phosphomevalonate kinase activity; ATP binding
Biological Process: isopentenyl diphosphate biosynthetic process, mevalonate pathway; sterol biosynthetic process; phosphorylation; cholesterol biosynthetic process
Research Articles on PMVK
Similar Products
Product Notes
The PMVK pmvk (Catalog #AAA175363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PMVK antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PMVK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the PMVK pmvk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMVK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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