Western Blot (WB)
(Figure 1. Western blot analysis of Presenilin 2/AD5/PSEN2 using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human HEK293 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 2/AD5/PSEN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753370) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Presenilin 2/AD5/PSEN2 at approximately 34KD,50KD. The expected band size for Presenilin 2/AD5/PSEN2 is at 50KD. )
Rabbit Presenilin 2/AD5/PSEN2 Polyclonal Antibody | anti-PSEN2 antibody
Anti-Presenilin 2/AD5/PSEN2 Antibody
IHC-P: 2-5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse, Rat|
Western Blot (WB)
(Figure 1. Western blot analysis of Presenilin 2/AD5/PSEN2 using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human HEK293 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 2/AD5/PSEN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753370) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Presenilin 2/AD5/PSEN2 at approximately 34KD,50KD. The expected band size for Presenilin 2/AD5/PSEN2 is at 50KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Presenilin 2/AD5/PSEN2 using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human HEK293 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 2/AD5/PSEN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753370) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Presenilin 2/AD5/PSEN2 at approximately 34KD,50KD. The expected band size for Presenilin 2/AD5/PSEN2 is at 50KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Overlay histogram showing HEPA1-6 cells stained with MBS1753370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 2/AD5/PSEN2 Antibody (MBS1753370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Overlay histogram showing HEPA1-6 cells stained with MBS1753370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 2/AD5/PSEN2 Antibody (MBS1753370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of RH-35 cells using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Overlay histogram showing RH-35 cells stained with MBS1753370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 2/AD5/PSEN2 Antibody (MBS1753370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of RH-35 cells using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Overlay histogram showing RH-35 cells stained with MBS1753370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 2/AD5/PSEN2 Antibody (MBS1753370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 4. Flow Cytometry analysis of U87 cells using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Overlay histogram showing U87 cells stained with MBS1753370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 2/AD5/PSEN2 Antibody (MBS1753370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 4. Flow Cytometry analysis of U87 cells using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Overlay histogram showing U87 cells stained with MBS1753370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 2/AD5/PSEN2 Antibody (MBS1753370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Immunofluorescence (IF)
(Figure 5. IF analysis of Presenilin 2/AD5/PSEN2 using anti- Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Presenilin 2/AD5/PSEN2 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Presenilin 2/AD5/PSEN2 Antibody (MBS1753370) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 5. IF analysis of Presenilin 2/AD5/PSEN2 using anti- Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Presenilin 2/AD5/PSEN2 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Presenilin 2/AD5/PSEN2 Antibody (MBS1753370) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
2. Piscopo, P., Marcon, G., Piras, M. R., Crestini, A., Campeggi, L. M., Deiana, E., Cherchi, R., Tanda, F., Deplano, A., Vanacore, N., Tagliavini, F., Pocchiari, M., Giaccone, G., Confaloni, A. A novel PSEN2 mutation associated with a peculiar phenotype. Neurology 70: 1549-1554, 2008.
3. Yu, C. -E., Marchani, E., Nikisch, G., Muller, U., Nolte, D., Hertel, A., Wijsman, E. M., Bird, T. D. The N141I mutation in PSEN2: implications for the quintessential case of Alzheimer disease. Arch. Neurol. 67: 631-633, 2010.
NCBI and Uniprot Product Information
NCBI Description
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
PSEN2: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). An integral membrane protein of the Golgi and endoplasmic reticulum. Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. Two alternative splice isoforms have been identified.
Protein type: Protease; Cell surface; Membrane protein, integral; EC 3.4.23.-; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q42.13
Cellular Component: Golgi apparatus; centrosome; cell surface; lysosomal membrane; integral to plasma membrane; nuclear inner membrane; cytosol; lipid raft; ciliary rootlet; kinetochore; membrane; cell soma; perinuclear region of cytoplasm; mitochondrial inner membrane; apical plasma membrane; dendritic shaft; neuromuscular junction; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cell cortex; Z disc; Golgi membrane; growth cone; axon; plasma membrane
Molecular Function: protein binding; endopeptidase activity; aspartic-type endopeptidase activity
Biological Process: positive regulation of catalytic activity; axon guidance; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; protein maturation; regulation of synaptic plasticity; positive regulation of coagulation; cell fate specification; T cell receptor signaling pathway; T cell activation during immune response; protein transport; amyloid precursor protein catabolic process; hair follicle development; negative regulation of protein amino acid phosphorylation; beta-amyloid metabolic process; calcium ion transport; forebrain development; ephrin receptor signaling pathway; brain morphogenesis; negative regulation of protein binding; dorsoventral neural tube patterning; embryonic limb morphogenesis; cardiac muscle contraction; Notch signaling pathway; somitogenesis; membrane protein intracellular domain proteolysis; thymus development; membrane protein ectodomain proteolysis; regulation of epidermal growth factor receptor activity; Notch receptor processing; memory; endoplasmic reticulum calcium ion homeostasis; response to hypoxia; myeloid leukocyte differentiation; protein processing; hemopoietic progenitor cell differentiation; alveolus development; negative regulation of protein complex assembly; negative regulation of apoptosis
Disease: Alzheimer Disease 4; Cardiomyopathy, Dilated, 1v
Research Articles on PSEN2
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Product Notes
The PSEN2 psen2 (Catalog #AAA1753370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Presenilin 2/AD5/PSEN2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Presenilin 2/AD5/PSEN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human, Mouse| IHC-P: 2-5ug/ml|Human| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse, Rat|. Researchers should empirically determine the suitability of the PSEN2 psen2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Presenilin 2/AD5/PSEN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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