Western Blot (WB)
(Anti-ALK antibody, MBS175323, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: JURKAT Cell Lysate )
Rabbit ALK Polyclonal Antibody | anti-ALK antibody
Anti-ALK antibody
Western Blot (WB)
(Anti-ALK antibody, MBS175323, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: JURKAT Cell Lysate )
Western Blot (WB)
(Anti-ALK antibody, MBS175323, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: JURKAT Cell Lysate )
Immunohistochemistry (IHC)
(Anti-ALK antibody, MBS175323, IHC(P)IHC(P): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti-ALK antibody, MBS175323, IHC(P)IHC(P): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti-ALK antibody, MBS175323, IHC(P)IHC(P): Human Rectal Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-ALK antibody, MBS175323, IHC(P)IHC(P): Human Rectal Cancer Tissue )
Background: ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246(cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
Uniprot Description
ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors
Protein type: Kinase, protein; EC 2.7.10.1; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Alk family
Chromosomal Location of Human Ortholog: 2p23
Cellular Component: protein complex; integral to plasma membrane
Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: regulation of apoptosis; cell proliferation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; activation of NF-kappaB transcription factor
Disease: Neuroblastoma, Susceptibility To, 3
Research Articles on ALK
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Product Notes
The ALK alk (Catalog #AAA175323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALK antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the ALK alk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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