Rabbit anti-Human, Mouse XLF/NHEJ1 Polyclonal Antibody | anti-NHEJ1 antibody

Anti-XLF/NHEJ1 Antibody

Cat. Num. AAA1753083

• Gene Names: NHEJ1; XLF
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: XLF/NHEJ1; Polyclonal Antibody; Anti-XLF/NHEJ1 Antibody; Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor; NHEJ1; XLF; anti-NHEJ1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 299

• Applicable Applications for anti-NHEJ1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse); IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human, Mouse); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human XLF/NHEJ1 recombinant protein (Position: M1-E273).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Immunofluorescence (IF)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of NHEJ1 using anti-NHEJ1 antibody.)

Related Product Information for anti-NHEJ1 antibody

Description: Rabbit IgG polyclonal antibody for XLF/NHEJ1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse.

Background: Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that in humans is encoded by the NHEJ1 gene. It is mapped to 2q35. Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

References

1. Callebaut, I., Malivert, L., Fischer, A., Mornon, J.-P., Revy, P., de Villartay, J.-P. Cernunnos interacts with the XRCC4-DNA-ligase IV complex and is homologous to the yeast nonhomologous end-joining factor Nej1. J. Biol. Chem. 281: 13857-13860, 2006.
2. Cantagrel, V., Lossi, A.-M., Lisgo, S., Missirian, C., Borges, A., Philip, N., Fernandez, C., Cardoso, C., Figarella-Branger, D., Moncla, A., Lindsay, S., Dobyns, W. B., Villard, L. Truncation of NHEJ1 in a patient with polymicrogyria. Hum. Mutat. 28: 356-364, 2007.
3. Dai, Y., Kysela, B., Hanakahi, L. A., Manolis, K., Riballo, E., Stumm, M., Harville, T. O., West, S. C., Oettinger, M. A., Jeggo, P. A. Nonhomologous end joining and V(D)J recombination require an additional factor. Proc. Nat. Acad. Sci. 100: 2462-2467, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 74734059
• NCBI GeneID: 79840
• UniProt Accession #: Q9H9Q4
• NCBI Official Full Name: Non-homologous end-joining factor 1
• NCBI Official Synonym Full Names: non-homologous end joining factor 1
• NCBI Official Symbol: NHEJ1
• NCBI Official Synonym Symbols: XLF
• NCBI Protein Information: non-homologous end-joining factor 1
• UniProt Protein Name: Non-homologous end-joining factor 1
• Protein Family: Non-homologous end-joining factor
• UniProt Gene Name: NHEJ1
• UniProt Synonym Gene Names: XLF
• UniProt Entry Name: NHEJ1_HUMAN

NCBI Description

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding

Biological Process: central nervous system development; B cell differentiation; response to ionizing radiation; positive regulation of ligase activity; double-strand break repair via nonhomologous end joining; DNA recombination; T cell differentiation

Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation