PCOLCE elisa kit

Human PCOLCE ELISA Kit

Cat. Num. AAA1752883

• Gene Names: PCOLCE; PCPE; PCPE1; PCPE-1
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: PCOLCE; Human PCOLCE ELISA Kit; Human PCOLCE; Procollagen C-endopeptidase enhancer 1; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer; PCPE1; PCOLCE elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human PCOLCE
• Sequence Length: 449

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCOLCE elisa kit

Principle of the Assay: The Picokine™ Human PCOLCE Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PCOLCE with a 96-well strip plate that is pre-coated with antibody specific for PCOLCE. The detection antibody is a biotinylated antibody specific for PCOLCE. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated monoclonal antibody from goat. The kit contains recombinant Human PCOLCE with immunogen: Expression system for standard: NSO, Immunogen sequence: Q26-D449. The kit is analytically validated with ready to use reagents. To measure Human PCOLCE, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PCOLCE in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PCOLCE in the sample.!!Background/Introduction: Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. It is mapped to 7q22.1. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 157653329
• NCBI GeneID: 5118
• NCBI Accession #: NP_002584.2
• NCBI GenBank Nucleotide #: NP_002584.2
• UniProt Accession #: Q15113
• NCBI Official Full Name: procollagen C-endopeptidase enhancer 1
• NCBI Official Synonym Full Names: procollagen C-endopeptidase enhancer
• NCBI Official Symbol: PCOLCE
• NCBI Official Synonym Symbols: PCPE; PCPE1; PCPE-1
• NCBI Protein Information: procollagen C-endopeptidase enhancer 1
• UniProt Protein Name: Procollagen C-endopeptidase enhancer 1
• Protein Family: Procollagen C-endopeptidase enhancer
• UniProt Gene Name: PCOLCE
• UniProt Synonym Gene Names: PCPE1; PCPE-1
• UniProt Entry Name: PCOC1_HUMAN

NCBI Description

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular matrix; extracellular space

Molecular Function: heparin binding; collagen binding; protein binding; protease activator activity

Biological Process: multicellular organismal development; proteolysis