Human Serpin C1/Antithrombin-III ELISA Kit | SERPINC1 elisa kit

Human Serpin C1/Antithrombin-III PicoKine Fast ELISA Kit

Cat. Num. AAA1752775

• Gene Names: SERPINC1; AT3; AT3D; ATIII; THPH7; ATIII-R2; ATIII-T1; ATIII-T2
• Reactivity: Human
• Synonyms: Serpin C1/Antithrombin-III; Human Serpin C1/Antithrombin-III PicoKine Fast ELISA Kit; human Serpin C1; Antithrombin-III; ATIII; Serpin C1; SERPINC1; AT3; PRO0309; SERPINC1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human SERPINC1
• Sequence Length: 464

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPINC1 elisa kit

Principle of the Assay: The Picokine™ Human SERPINC1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SERPINC1 with a 96-well strip plate that is pre-coated with antibody specific for SERPINC1. The detection antibody is a biotinylated antibody specific for SERPINC1. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human SERPINC1 with immunogen: NSO, H33-K464. The kit is analytically validated with ready to use reagents. To measure Human SERPINC1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human SERPINC1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SERPINC1 in the sample.!!Background/Introduction: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).

NCBI and Uniprot Product Information

• NCBI GI #: 113936
• NCBI GeneID: 462
• UniProt Accession #: P01008
• NCBI Official Full Name: Antithrombin-III
• NCBI Official Synonym Full Names: serpin family C member 1
• NCBI Official Symbol: SERPINC1
• NCBI Official Synonym Symbols: AT3; AT3D; ATIII; THPH7; ATIII-R2; ATIII-T1; ATIII-T2
• NCBI Protein Information: antithrombin-III
• UniProt Protein Name: Antithrombin-III
• Protein Family: Antithrombin
• UniProt Gene Name: SERPINC1
• UniProt Synonym Gene Names: AT3; ATIII
• UniProt Entry Name: ANT3_HUMAN

NCBI Description

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

Uniprot Description

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protein binding; protease binding

Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient

Disease: Antithrombin Iii Deficiency