Rabbit JAK2 Monoclonal Antibody | anti-JAK2 antibody

Anti-JAK2 Monoclonal Antibody

Cat. Num. AAA1751903

• Gene Names: JAK2; JTK10; THCYT3
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity-Chromatography
• Synonyms: JAK2; Monoclonal Antibody; Anti-JAK2 Monoclonal Antibody; anti-JAK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Affinity-Chromatography
• Form/Format: Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1,132

• Applicable Applications for anti-JAK2 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:50-1:200
• Immunogen: A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term; Avoid freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent analysis of Jurkat cells, using JAK2 Antibody.)

Related Product Information for anti-JAK2 antibody

Rabbit IgG Monoclonal antibody for O60674 detection. Tested positive for WB IF/IF in Human Mouse Rat

NCBI and Uniprot Product Information

• NCBI GI #: 1015809690
• NCBI GeneID: 3717
• NCBI Accession #: NP_001309123.1
• NCBI GenBank Nucleotide #: NM_001322194.1
• UniProt Accession #: O60674; O14636; O75297
• Molecular Weight: 130,674 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK2 isoform a
• NCBI Official Synonym Full Names: Janus kinase 2
• NCBI Official Symbol: JAK2
• NCBI Official Synonym Symbols: JTK10; THCYT3
• NCBI Protein Information: tyrosine-protein kinase JAK2
• UniProt Protein Name: Tyrosine-protein kinase JAK2
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK2
• UniProt Synonym Gene Names: JAK-2

NCBI Description

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980).