Rabbit PU.1 Polyclonal Antibody | anti-PU.1 antibody

PU.1 (R212) Polyclonal Antibody

Cat. Num. AAA3002890

• Gene Names: SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PU.1; Polyclonal Antibody; PU.1 (R212) Polyclonal Antibody; SPI1; SFPI1; Transcription factor PU.1; 31 kDa-transforming protein; anti-PU.1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PU.1 (R212) polyclonal antibody detects endogenous levels of PU.1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 271

• Applicable Applications for anti-PU.1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human PU.1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PU. 1 (R212) polyclonal antibody at 1:1000 dilution Lane1:JURKAT whole cell lysate (37ug) Lane2:THP-1 whole cell lysate (37ug) Lane3:The blood cell tissue lysate of Rat (36ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PU. 1 (R212) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PU. 1 (R212) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PU. 1 (R212) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PU. 1 (R212) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of PU. 1 (R212) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug))

Related Product Information for anti-PU.1 antibody

The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T lineage commitment and then is dramatically absent following the beta selection checkpoint.

NCBI and Uniprot Product Information

• NCBI GI #: 124028521
• NCBI GeneID: 6688
• NCBI Accession #: NP_001074016.1
• NCBI GenBank Nucleotide #: NP_001074016.1
• UniProt Accession #: P17947
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: transcription factor PU.1 isoform 1
• NCBI Official Synonym Full Names: Spi-1 proto-oncogene
• NCBI Official Symbol: SPI1
• NCBI Official Synonym Symbols: OF; PU.1; SFPI1; SPI-1; SPI-A
• NCBI Protein Information: transcription factor PU.1
• UniProt Protein Name: Transcription factor PU.1
• UniProt Gene Name: SPI1
• UniProt Entry Name: SPI1_HUMAN

NCBI Description

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; RNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; macrophage differentiation; granulocyte differentiation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; lymphoid progenitor cell differentiation; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of transcription from RNA polymerase II promoter; regulation of erythrocyte differentiation; lymphocyte differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent