Human Nectin-2/CD112 ELISA Kit | CD112 elisa kit
Human Nectin-2/CD112 PicoKine ELISA Kit
Gene Names
NECTIN2; PRR2
Reactivity
Human
Synonyms
Nectin-2/CD112; Human Nectin-2/CD112 PicoKine ELISA Kit; Human Nectin-2/CD112; CD112 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Nectin-2/CD112
Sequence Length
479
Assay Range
156pg/ml-10000pg/ml
Sensitivity
<0.1ng/ml
Capture Antibody
A monoclonal antibody from mouse.
Detection Antibody
A biotinylated detection polyclonal antibody from goat.
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |211|1599|5167|264|1794|5274
Standard Deviation: |9.92|84.75|289.35|17.69|91.49|253.15
CV(%): |4.7%|5.3%|5.6%|6.7%|5.1%|4.8%
n|16|16|16|24|24|24
Mean(pg/ml): |211|1599|5167|264|1794|5274
Standard Deviation: |9.92|84.75|289.35|17.69|91.49|253.15
CV(%): |4.7%|5.3%|5.6%|6.7%|5.1%|4.8%
Sample Data
Concentration (pg/ml): |0|156|312|625|1250|2500|5000|10000
OD: |0.043|0.162|0.176|0.258|0.449|0.857|1.573|2.181
OD: |0.043|0.162|0.176|0.258|0.449|0.857|1.573|2.181
Related Product Information for CD112 elisa kit
Description: For quantitative detection of human Nectin-2/CD112 in cell culture supernates, serum and plasma (heparin, EDTA).
Background: Human Nectin-2/CD112 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nectin-2/CD112 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q32-G360) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nectin-2/CD112 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Poliovirus receptor-related 2 (PVRL2), also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), is a human plasma membrane glycoprotein. It is mapped to 19q13.32. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Background: Human Nectin-2/CD112 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nectin-2/CD112 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q32-G360) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nectin-2/CD112 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Poliovirus receptor-related 2 (PVRL2), also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), is a human plasma membrane glycoprotein. It is mapped to 19q13.32. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nectin-2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
PRR2
NCBI Protein Information
nectin-2
Research Articles on CD112
Similar Products
Product Notes
The Human CD112 (Catalog #AAA1751704) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751704 ELISA Kit recognizes Human CD112. It is sometimes possible for the material contained within the vial of "Nectin-2/CD112, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
