Testing Data
Nectin-2 ELISA Pair Set | NECTIN2 elisa pair set
Nectin-2 Matched ELISA Antibody Pair Set, Human
Synonyms
Nectin-2; Nectin-2 Matched ELISA Antibody Pair Set; Human; Human CD112/Nectin-2/PVRL2 ELISA Pair Set; poliovirus receptor-related 2 (herpesvirus entry mediator B); CD112 Matched ELISA Antibody Pair Set; HVEB Matched ELISA Antibody Pair Set; PRR2 Matched ELISA Antibody Pair Set; PVRR2 Matched ELISA Antibody Pair Set; NECTIN2 elisa pair set
Specificity
Quantitative determination of Human Nectin-2
Assay Type
Solid Phase Sandwich ELISA
Detection Range
15.625-1000pg/mL
Sensitivity
15.625pg/ml
Materials Provided
1. Capture Antibody 0.25 mg/mL of rabbit anti-CD112 monoclonal antibody, Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-CD112 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 50 ng of recombinant CD112. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-CD112 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 50 ng of recombinant CD112. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for NECTIN2 elisa pair set
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell-and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
This Nectin-2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Nectin-2. It contains Human Nectin-2 capture antibody, Human Nectin-2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Nectin-2 protein.
This Nectin-2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Nectin-2. It contains Human Nectin-2 capture antibody, Human Nectin-2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Nectin-2 protein.
References
Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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Product Notes
The NECTIN2 (Catalog #AAA8118170) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nectin-2, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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