Human Cathepsin V ELISA Kit | CTSV elisa kit
Human Cathepsin V PicoKine ELISA Kit
Gene Names
CTSV; CTSU; CATL2; CTSL2
Reactivity
Human
Synonyms
Cathepsin V; Human Cathepsin V PicoKine ELISA Kit; Human Cathepsin V; CTSV elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Cathepsin V
Sequence Length
334
Assay Range
78pg/ml-5000pg/ml
Sensitivity
<15pg/ml
Capture Antibody
A monoclonal antibody from mouse.
Detection Antibody
A biotinylated detection polyclonal antibody from goat.
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |100|714|2346|136|823|2464
Standard Deviation: |5.30|44.27|105.57|6.39|52.67|133.06
CV(%): |5.3%|6.2%|4.5%|4.7%|6.4%|5.4%
n|16|16|16|24|24|24
Mean(pg/ml): |100|714|2346|136|823|2464
Standard Deviation: |5.30|44.27|105.57|6.39|52.67|133.06
CV(%): |5.3%|6.2%|4.5%|4.7%|6.4%|5.4%
Sample Data
Concentration (pg/ml): |0|78|156|312|625|1250|2500|5000
OD: |0.096|0.132|0.216|0.379|0.609|1.011|1.828|2.542
OD: |0.096|0.132|0.216|0.379|0.609|1.011|1.828|2.542
Related Product Information for CTSV elisa kit
Description: For quantitative detection of human Cathepsin V in cell culture supernates, serum and plasma (heparin, EDTA).
Background: Human Cathepsin V ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin V has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: V18-V334) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin V is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.
Background: Human Cathepsin V ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin V has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: V18-V334) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin V is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2011]
Research Articles on CTSV
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Product Notes
The Human CTSV (Catalog #AAA1751679) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751679 ELISA Kit recognizes Human CTSV. It is sometimes possible for the material contained within the vial of "Cathepsin V, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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