Rabbit DCI Polyclonal Antibody | anti-ECI1 antibody

Anti-DCI antibody

Cat. Num. AAA175111

• Gene Names: ECI1; DCI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: DCI; Polyclonal Antibody; Anti-DCI antibody; Enoyl-CoA delta isomerase 1; mitochondrial; enoyl-CoA delta isomerase 1; 3; 2 trans enoyl Coenzyme A isomerase antibody; Acetylene allene isomerase antibody; D3; D2 enoyl CoA isomerase antibody; DCI antibody; DCI protein antibody; Delta(3); Delta(2) enoyl CoA isomerase antibody; Delta3; delta2 enoyl CoA isomerase antibody; Dodecenoyl CoA delta Isomerase antibody; Dodecenoyl CoA isomerase antibody; Dodecenoyl Coenzyme A delta isomerase antibody; Dodecenoyl Coenzyme A delta isomerase(3; 2 trans enoyl Coenzyme A isomerase) antibody; Dodecenoyl Coenzyme A delta isomerase 3; 2 trans enoyl Coenzyme A isomerase; isoform CRA a antibody; anti-ECI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 302

• Applicable Applications for anti-ECI1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DCI (272-290aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-DCI antibody, MBS175111, Western blottingAll lanes: Anti DCI (MBS175111) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD )

Immunohistochemistry (IHC)

(Anti-DCI antibody, MBS175111, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Immunocytochemistry (ICC)

(Anti-DCI antibody, MBS175111, ICCICC: HELA Cell )

Immunocytochemistry (ICC)

(Anti-DCI antibody, MBS175111, ICCICC: A549 Cell )

Related Product Information for anti-ECI1 antibody

Description: Rabbit IgG polyclonal antibody for Enoyl-CoA delta isomerase 1, mitochondrial(ECI1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon(position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

NCBI and Uniprot Product Information

• NCBI GI #: 1169204
• NCBI GeneID: 1632
• UniProt Accession #: P42126; Q13290; Q7Z2L6; Q7Z2L7; Q9BUB8; Q9BW05; Q9UDG6; A8K512
• Molecular Weight: 30,896 Da
• NCBI Official Full Name: Enoyl-CoA delta isomerase 1, mitochondrial
• NCBI Official Synonym Full Names: enoyl-CoA delta isomerase 1
• NCBI Official Symbol: ECI1
• NCBI Official Synonym Symbols: DCI
• NCBI Protein Information: enoyl-CoA delta isomerase 1, mitochondrial; dodecenoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; acetylene-allene isomerase; 3,2 trans-enoyl-CoA isomerase; delta3, delta2-enoyl-CoA isomerase; 3,2 trans-enoyl-Coenzyme A isomerase; delta(3),Delta(2)-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
• UniProt Protein Name: Enoyl-CoA delta isomerase 1, mitochondrial
• Protein Family: Enoyl-CoA delta isomerase
• UniProt Gene Name: ECI1
• UniProt Synonym Gene Names: DCI; D3,D2-enoyl-CoA isomerase
• UniProt Entry Name: ECI1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Uniprot Description

DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.3.3.8; Mitochondrial; Isomerase; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process