TGF-Beta 3 ELISA Kit | TGFB3 elisa kit

Chicken TGF-Beta 3 PicoKine ELISA Kit

Cat. Num. AAA1751098

• Gene Names: TGFB3; ARVD; LDS5; RNHF; ARVD1; TGF-beta3
• Reactivity: Chicken; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: TGF-Beta 3; Chicken TGF-Beta 3 PicoKine ELISA Kit; Transforming growth factor beta-3; TGF-beta-3; Latency-associated peptide; LAP; TGFB3; TGFB3 elisa kit

• Reactivity: Chicken; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Chicken Chicken TGF-Beta 3
• Sequence Length: 309

• Applicable Applications for TGFB3 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFB3 elisa kit

Principle of the Assay: Chicken Chicken TGF-Beta 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chicken TGF-Beta 3 has been precoated onto 96-well plates. Standards(Expression system for standard: sf21; Immunogen sequence: A301-S412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chicken TGF-Beta 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Chicken Chicken TGF-Beta 3 amount of sample captured in plate.

Background: Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.

Product Categories/Family for TGFB3 elisa kit

Signal Transduction; Growth Factors/Hormones; TGF; Stem Cells; Signaling Pathways; TGF Beta; Secreted; Cancer; Growth Factors; Invasion/Microenvironment; Angiogenesis; Angiogenic Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1050108310
• NCBI GeneID: 7043
• NCBI Accession #: NP_001316867.1
• NCBI GenBank Nucleotide #: NM_001329938.1
• UniProt Accession #: P10600; Q8WV88
• Molecular Weight: 47328 MW
• NCBI Official Full Name: transforming growth factor beta-3 isoform 2
• NCBI Official Synonym Full Names: transforming growth factor beta 3
• NCBI Official Symbol: TGFB3
• NCBI Official Synonym Symbols: ARVD; LDS5; RNHF; ARVD1; TGF-beta3
• NCBI Protein Information: transforming growth factor beta-3
• UniProt Protein Name: Transforming growth factor beta-3
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFB3
• UniProt Synonym Gene Names: TGF-beta-3; LAP

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing. Mutations in this gene are a cause of aortic aneurysms and dissections, as well as familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Aug 2016]

Uniprot Description

Involved in embryogenesis and cell differentiation.