Western Blot (WB)
(Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (MBS1750745).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TREM1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD. )
Rabbit TREM1 Polyclonal Antibody | anti-TREM1 antibody
Anti-TREM1 Picoband Antibody
Gene Names
TREM1; CD354; TREM-1
Reactivity
HumanNo cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
TREM1; Polyclonal Antibody; Anti-TREM1 Picoband Antibody; Triggering receptor expressed on myeloid cells 1; TREM-1; Triggering receptor expressed on monocytes 1; CD354; anti-TREM1 antibody
Host
Rabbit
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified
Form/Format
Lyophilized
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (varies by lot)
Sequence Length
225
Applicable Applications for anti-TREM1 antibody
Western Blot (WB)
Application Notes
WB: 0.1-0.5mug/ml
Immunogen
E Coli-derived human TREM1 recombinant protein (Position: A21-R200).
Subcellular Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (MBS1750745).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TREM1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (MBS1750745).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TREM1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD. )
Related Product Information for anti-TREM1 antibody
Description: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
Protein Function: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.
Protein Function: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.
Product Categories/Family for anti-TREM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,574 Da
NCBI Official Full Name
triggering receptor expressed on myeloid cells 1 isoform 2
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 1
NCBI Official Symbol
TREM1
NCBI Official Synonym Symbols
CD354; TREM-1
NCBI Protein Information
triggering receptor expressed on myeloid cells 1
UniProt Protein Name
Triggering receptor expressed on myeloid cells 1
Protein Family
UniProt Gene Name
TREM1
UniProt Synonym Gene Names
TREM-1
NCBI Description
This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
Uniprot Description
Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.
Research Articles on TREM1
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Product Notes
The TREM1 trem1 (Catalog #AAA1750745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TREM1 Picoband Antibody reacts with Human No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's TREM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.1-0.5mug/ml. Researchers should empirically determine the suitability of the TREM1 trem1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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