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Alkaline Phosphatase Active Protein | ALPI active protein

Alkaline Phosphatase (CIP/CIAP), Calf Intestinal

Purity
Purified
Synonyms
Alkaline Phosphatase; Alkaline Phosphatase (CIP/CIAP); Calf Intestinal; Alk Phos; CIP; CIAP; ALP; Orthophosphoric-Monoester Phosphohydrolase; Calf Intestinal - Lyophilized; ALPI active protein
Ordering
For Research Use Only!
Purity/Purification
Purified
Form/Format
Lyophilized powder with magnesium chloride.
Sequence Length
524
Source
Calf Intestines
EC Number
3.1.3.1
Unit Definition
One unit will convert on micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37 degree C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.25 and 37 degree C.
Specific Activity
1390 units/mg protein
Appearance
Tan powder
Solubility
Clear, colorless in 1 mg/mL saline.
Protein
0.48 mg protein/mg (Coomassie)
Contaminants
Ammonia: <0.1 micromole/mg
CAS Number
9001-78-9
Activity
660 Units/mg (Dimension Clinical Chemistry System)
Reconstitution
Tris buffered saline with 1 mM magnesium chloride, 0.2mM zinc chloride, and 1% BSA, pH8.0
Preparation and Storage
Aliquot and freeze at -20 degree C after reconstitution. Avoid freeze thaw cycles.
Note: Protect from moisture
Advice: Reconstitute into provided container and centrifuge to ensure full recovery.
Handling: Handle as a potentially hazardous substance.
Related Product Information for ALPI active protein
MyBioSource is the leading producer of Calf Intestine Alkaline Phosphatase (CIAP, ALP, ALKP) for research.

Custom preparations, technical support, bulk quantities and aliquoting available.

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alkaline phosphatase, tissue-nonspecific isozyme
NCBI Official Symbol
ALPL
NCBI Protein Information
alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; AP-TNAP; alkaline phosphatase liver/bone/kidney isozyme
UniProt Protein Name
Alkaline phosphatase, tissue-nonspecific isozyme
Protein Family
UniProt Gene Name
ALPL
UniProt Synonym Gene Names
AP-TNAP; TNSALP
UniProt Entry Name
PPBT_FELCA

Uniprot Description

This isozyme may play a role in skeletal mineralization.

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Product Notes

The ALPI alpl (Catalog #AAA173171) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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