Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit | CYP3A5 elisa kit

Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit

Cat. Num. AAA166915

• Gene Names: CYP3A5; CP35; PCN3; CYPIIIA5; P450PCN3
• Reactivity: Human
• Synonyms: Cytochrome P450 3A5 (CYP3A5); Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit; CYP3A5 elisa kit

• Reactivity: Human
• Sequence Length: 492

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.3ng/ml - 90ng/ml
• Sensitivity: 0.14ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP3A5 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Cytochrome P450 3A5 (also known as CYP3A5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CYP3A5 antibody. CYP3A5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CYP3A5 Antibody is added and binds to CYP3A5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP3A5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CYP3A5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 628601851
• NCBI GeneID: 1577
• NCBI Accession #: NP_001278759.1
• NCBI GenBank Nucleotide #: NM_001291830.1
• Molecular Weight: 15,846 Da
• NCBI Official Full Name: cytochrome P450 3A5 isoform 4
• NCBI Official Synonym Full Names: cytochrome P450 family 3 subfamily A member 5
• NCBI Official Symbol: CYP3A5
• NCBI Official Synonym Symbols: CP35; PCN3; CYPIIIA5; P450PCN3
• NCBI Protein Information: cytochrome P450 3A5
• UniProt Protein Name: Cytochrome P450 3A5
• UniProt Gene Name: CYP3A5

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.MiscellaneousChimeric transcripts, characterized by CYP3A43 exon 1 joined at canonical splice sites to distinct sets of CYP3A5 exons, have been detected. All are possibly produced by trans-splicing. The chimeric transcripts exist in 2 different combinations: CYP3A43 exon 1 joined in frame to CYP3A5 exon 11-13 and CYP3A43 exon 1 joined in frame to CYP3A5 exon 12-13. All chimeric transcripts are expressed at very low levels in the liver (PubMed:11726664).