Typical Testing Data/Standard Curve (for reference only)
Human Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha) ELISA Kit | PGC1alpha elisa kit
Human Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha) ELISA Kit
Gene Names
Ppargc1a; Ppargc1
Reactivity
Human
Synonyms
Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha); Human Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha) ELISA Kit; PGC1alpha elisa kit
Reactivity
Human
Sequence Length
796
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.2ng/ml-70ng/ml
Sensitivity
0.097ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PGC1alpha elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Peroxisome Proliferator Activated Recepter Beta (also known as PPAR-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human PPAR-B antibody. PPAR-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human PPAR-B Antibody is added and binds to PPAR-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPAR-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human PPAR-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human PPAR-B antibody. PPAR-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human PPAR-B Antibody is added and binds to PPAR-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPAR-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human PPAR-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90,622 Da
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
NCBI Official Synonym Full Names
PPARG coactivator 1 alpha
NCBI Official Symbol
Ppargc1a
NCBI Official Synonym Symbols
Ppargc1
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Gene Name
Ppargc1a
UniProt Synonym Gene Names
Pgc1; Pgc1a; Ppargc1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
NCBI Description
nuclear receptor coactivator; may mediate glucose homeostasis, and lipid homeostasis [RGD, Feb 2006]
Uniprot Description
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK ().
Research Articles on PGC1alpha
Similar Products
Product Notes
The Human PGC1alpha ppargc1a (Catalog #AAA166855) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166855 ELISA Kit recognizes Human PGC1alpha. It is sometimes possible for the material contained within the vial of "Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
