Rabbit anti-Human TBC1D4 Polyclonal Antibody | anti-TBC1D4 antibody

TBC1D4 Antibody

Cat. Num. AAA9411013

• Gene Names: TBC1D4; AS160; NIDDM5
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: TBC1D4; Polyclonal Antibody; TBC1D4 Antibody; AS160; anti-TBC1D4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total TBC1D4 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 2.6 mg/ml (varies by lot)
• Sequence Length: 1290

• Applicable Applications for anti-TBC1D4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:30-1:150
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human TBC1 domain family, member 4
• Target Name: TBC1D4
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): Hela and hepg2 cellAmount of lysate: 40ug per lane Primary antibody: 1/320 dilution Secondary antibody dilution: 1/8000Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/40.)

Related Product Information for anti-TBC1D4 antibody

TBC1 domain family member 4 (TBC1D4), also designated AS160, can be insulin- and/or AKT1-induced. Insulin-stimulated phosphorylation is required for GLUT4 translocation. TBC1D4 may play a role as a GTPase activating protein for proteins in the Rab family. It is expressed primarily in skeletal muscle and heart, as well as spleen, lymph node and leukocytes. Defects in the TBC1D4 gene may cause atopic dermatitis (AD), sometimes referred to as eczema, an atopic chronic skin disease. The skin of affected individuals reacts to irritants or allergens and becomes red, flaky and itchy. The skin is also more vulnerable to inflammations, and symptoms can grow or disappear over time.

Product Categories/Family for anti-TBC1D4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 557786188
• NCBI GeneID: 9882
• NCBI Accession #: NP_001273587.1
• NCBI GenBank Nucleotide #: NM_001286658.2
• UniProt Accession #: O60343; Q5W0B9; Q68D14; A7E2X8; B4DU25; B4E235; B6ETN8; B6ETN9
• Molecular Weight: 60,095 Da
• NCBI Official Full Name: TBC1 domain family member 4 isoform 2
• NCBI Official Synonym Full Names: TBC1 domain family, member 4
• NCBI Official Symbol: TBC1D4
• NCBI Official Synonym Symbols: AS160; NIDDM5
• NCBI Protein Information: TBC1 domain family member 4
• UniProt Protein Name: TBC1 domain family member 4
• Protein Family: TBC1 domain family
• UniProt Gene Name: TBC1D4
• UniProt Synonym Gene Names: AS160; KIAA0603; AS160
• UniProt Entry Name: TBCD4_HUMAN

NCBI Description

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.