Typical Testing Data/Standard Curve (for reference only)
Human Krueppel-like factor 5 (KLF5) ELISA Kit | KLF5 elisa kit
Human Krueppel-like factor 5 (KLF5) ELISA Kit
Gene Names
KLF5; CKLF; IKLF; BTEB2
Reactivity
Human
Synonyms
Krueppel-like factor 5 (KLF5); Human Krueppel-like factor 5 (KLF5) ELISA Kit; KLF5 elisa kit
Reactivity
Human
Sequence Length
457
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.1ng/ml - 40ng/ml
Sensitivity
0.059ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KLF5 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Krueppel-like Factor 5 (also known as KLF5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human KLF5 antibody. KLF5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human KLF5 Antibody is added and binds to KLF5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KLF5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human KLF5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human KLF5 antibody. KLF5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human KLF5 Antibody is added and binds to KLF5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KLF5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human KLF5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,618 Da
NCBI Official Full Name
Krueppel-like factor 5 isoform 1
NCBI Official Synonym Full Names
Kruppel like factor 5
NCBI Official Symbol
KLF5
NCBI Official Synonym Symbols
CKLF; IKLF; BTEB2
NCBI Protein Information
Krueppel-like factor 5
UniProt Protein Name
Krueppel-like factor 5
Protein Family
UniProt Gene Name
KLF5
UniProt Synonym Gene Names
BTEB2; CKLF; IKLF; BTE-binding protein 2
NCBI Description
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Uniprot Description
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Research Articles on KLF5
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Product Notes
The Human KLF5 klf5 (Catalog #AAA165842) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA165842 ELISA Kit recognizes Human KLF5. It is sometimes possible for the material contained within the vial of "Krueppel-like factor 5 (KLF5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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