Typical Testing Data/Standard Curve (for reference only)
Human Inter Alpha-trypsin Inhibitor H3 (ITIH3) ELISA Kit | ITIH3 elisa kit
Human Inter Alpha-trypsin Inhibitor H3 (ITIH3) ELISA Kit
Gene Names
ITIH3; H3P; SHAP; ITI-HC3
Reactivity
Human
Synonyms
Inter Alpha-trypsin Inhibitor H3 (ITIH3); Human Inter Alpha-trypsin Inhibitor H3 (ITIH3) ELISA Kit; ITIH3 elisa kit
Reactivity
Human
Sequence Length
890
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
5ng/ml - 1500ng/ml
Sensitivity
2.42ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ITIH3 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Inter Alpha-trypsin Inhibitor H3 (also known as ITIH3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ITIH3 antibody. ITIH3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ITIH3 Antibody is added and binds to ITIH3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ITIH3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ITIH3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ITIH3 antibody. ITIH3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ITIH3 Antibody is added and binds to ITIH3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ITIH3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ITIH3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,328 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 3
NCBI Official Symbol
ITIH3
NCBI Official Synonym Symbols
H3P; SHAP; ITI-HC3
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H3
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H3
Protein Family
UniProt Gene Name
ITIH3
UniProt Synonym Gene Names
ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP
NCBI Description
This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]
Uniprot Description
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Research Articles on ITIH3
Similar Products
Product Notes
The Human ITIH3 itih3 (Catalog #AAA165152) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA165152 ELISA Kit recognizes Human ITIH3. It is sometimes possible for the material contained within the vial of "Inter Alpha-trypsin Inhibitor H3 (ITIH3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
