Human Caspase 14 (CASP14) ELISA Kit | CASP14 elisa kit

Human Caspase 14 (CASP14) ELISA Kit

Cat. Num. AAA165144

• Gene Names: CASP14; ARCI12
• Reactivity: Human
• Synonyms: Caspase 14 (CASP14); Human Caspase 14 (CASP14) ELISA Kit; CASP14 elisa kit

• Reactivity: Human
• Sequence Length: 242

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 20ng/L - 6000ng/L
• Sensitivity: 10.33ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP14 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Caspase-14 (also known as CASP14) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CASP14 antibody. CASP14 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CASP14 Antibody is added and binds to CASP14 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CASP14 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CASP14. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 6912286
• NCBI GeneID: 23581
• NCBI Accession #: NP_036246.1
• NCBI GenBank Nucleotide #: NM_012114.2
• UniProt Accession #: P31944; O95823; Q3SYC9
• Molecular Weight: 27,680 Da
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14
• NCBI Official Symbol: CASP14
• NCBI Official Synonym Symbols: ARCI12
• NCBI Protein Information: caspase-14
• UniProt Protein Name: Caspase-14
• Protein Family: Caspase
• UniProt Gene Name: CASP14
• UniProt Synonym Gene Names: CASP-14

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).