Typical Testing Data/Standard Curve (for reference only)
Human Leukocyte Elastase Inhibitor (LEI) ELISA Kit | LEI elisa kit
Human Leukocyte Elastase Inhibitor (LEI) ELISA Kit
Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
Reactivity
Human
Synonyms
Leukocyte Elastase Inhibitor (LEI); Human Leukocyte Elastase Inhibitor (LEI) ELISA Kit; LEI elisa kit
Reactivity
Human
Sequence Length
379
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
10pg/ml-3000pg/ml
Sensitivity
5.12pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LEI elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human L-Dihydroxyphenyalanine (also known as L-DOPA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human L-DOPA antibody. L-DOPA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human L-DOPA Antibody is added and binds to L-DOPA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated L-DOPA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human L-DOPA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human L-DOPA antibody. L-DOPA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human L-DOPA Antibody is added and binds to L-DOPA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated L-DOPA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human L-DOPA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,073 Da
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin family B member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
NCBI Protein Information
leukocyte elastase inhibitor
UniProt Protein Name
Leukocyte elastase inhibitor
Protein Family
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
NCBI Description
The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also functions as a potent intracellular inhibitor of granzyme H.
Research Articles on LEI
Similar Products
Product Notes
The Human LEI serpinb1 (Catalog #AAA162214) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA162214 ELISA Kit recognizes Human LEI. It is sometimes possible for the material contained within the vial of "Leukocyte Elastase Inhibitor (LEI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
