Human ATP-binding cassette sub-family G member 5, ABCG5 ELISA Kit | ABCG5 elisa kit

Human ATP-binding cassette sub-family G member 5, ABCG5 ELISA Kit

Cat. Num. AAA1607381

• Gene Names: ABCG5; STSL
• Reactivity: Human
• Synonyms: ATP-binding cassette sub-family G member 5; ABCG5; Human ATP-binding cassette sub-family G member 5; ABCG5 ELISA Kit; ABCG5 elisa kit

• Reactivity: Human
• Sequence Length: 651

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 20ng/ml
• Sensitivity: 0.024ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCG5 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human ATP-binding Cassette Sub-family G Member 5 (also known as ABCG5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ABCG5 antibody. ABCG5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ABCG5 Antibody is added and binds to ABCG5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCG5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ABCG5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 11967969
• NCBI GeneID: 64240
• NCBI GenBank Nucleotide #: NP_071881.1
• UniProt Accession #: Q9H222
• NCBI Official Full Name: ATP-binding cassette sub-family G member 5
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 5
• NCBI Official Symbol: ABCG5
• NCBI Official Synonym Symbols: STSL
• NCBI Protein Information: ATP-binding cassette sub-family G member 5
• UniProt Protein Name: ATP-binding cassette sub-family G member 5
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG5
• UniProt Entry Name: ABCG5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG5: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Defects in ABCG5 are a cause of sitosterolemia (STSL); also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Protein type: Membrane protein, integral; Transporter, ABC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: apical plasma membrane; plasma membrane; ATP-binding cassette (ABC) transporter complex; receptor complex

Molecular Function: protein binding; protein heterodimerization activity; cholesterol transporter activity; ATPase activity; ATP binding

Biological Process: response to drug; negative regulation of cholesterol absorption; cholesterol homeostasis; cholesterol absorption; cholesterol efflux; response to ionizing radiation; excretion; transmembrane transport; response to nutrient; sterol transport

Disease: Sitosterolemia