Typical Testing Data/Standard Curve (for reference only)
Human Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, MTHFD2 ELISA Kit | MTHFD2 elisa kit
Human Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, MTHFD2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MTHFD2 antibody. MTHFD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MTHFD2 Antibody is added and binds to MTHFD2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MTHFD2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MTHFD2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
Uniprot Description
MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.15; EC 3.5.4.9; Hydrolase; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: cytosol; extracellular space; mitochondrial matrix; mitochondrion
Molecular Function: formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; phosphate binding
Biological Process: folic acid metabolic process; one-carbon compound metabolic process; tetrahydrofolate metabolic process
Research Articles on MTHFD2
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Product Notes
The Human MTHFD2 mthfd2 (Catalog #AAA1607306) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1607306 ELISA Kit recognizes Human MTHFD2. It is sometimes possible for the material contained within the vial of "Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, MTHFD2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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