Human Choline/ethanolamine kinase, CHKB ELISA Kit | CHKB elisa kit

Human Choline/ethanolamine kinase, CHKB ELISA Kit

Cat. Num. AAA1605974

• Gene Names: CHKB; CK; EK; CKB; EKB; CHKL; CHETK; CKEKB; MDCMC
• Reactivity: Human
• Synonyms: Choline/ethanolamine kinase; CHKB; Human Choline/ethanolamine kinase; CHKB ELISA Kit; CHKB elisa kit

• Reactivity: Human
• Sequence Length: 395

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15-3000ng/L
• Sensitivity: 7.55ng/L
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHKB elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Choline; ethanolamine kinase (also known as CHKB) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CHKB antibody. CHKB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CHKB Antibody is added and binds to CHKB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHKB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CHKB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 6978649
• NCBI GeneID: 1120
• NCBI GenBank Nucleotide #: NP_005189.2
• UniProt Accession #: Q9Y259
• NCBI Official Full Name: choline/ethanolamine kinase
• NCBI Official Synonym Full Names: choline kinase beta
• NCBI Official Symbol: CHKB
• NCBI Official Synonym Symbols: CK; EK; CKB; EKB; CHKL; CHETK; CKEKB; MDCMC
• NCBI Protein Information: choline/ethanolamine kinase
• UniProt Protein Name: Choline/ethanolamine kinase
• Protein Family: Choline/ethanolamine kinase
• UniProt Gene Name: CHKB
• UniProt Synonym Gene Names: CHETK; CHKL; CK; CKB; EK; EKB; CKEKB
• UniProt Entry Name: CHKB_HUMAN

NCBI Description

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]

Uniprot Description

CHKB: Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; EC 2.7.1.82; Lipid Metabolism - glycerophospholipid; EC 2.7.1.32

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: choline kinase activity; ethanolamine kinase activity; ATP binding

Biological Process: CDP-choline pathway; phospholipid metabolic process; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; phosphorylation

Disease: Muscular Dystrophy, Congenital, Megaconial Type