Typical Testing Data/Standard Curve (for reference only)
Canine Melanoma-associated Antigen D4 ELISA Kit | MAGED4 elisa kit
Canine Melanoma-associated Antigen D4 ELISA Kit
Gene Names
MAGED4; MAGE1; MAGEE1; MAGE-D4; MAGE-E1
Reactivity
Canine
Synonyms
Melanoma-associated Antigen D4; Canine Melanoma-associated Antigen D4 ELISA Kit; MAGED4 elisa kit
Reactivity
Canine
Sequence Length
741
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.5ng/ml-100ng/ml
Sensitivity
0.29ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MAGED4 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Intestinal Trefoil Factor (also known as ITF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine ITF antibody. ITF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine ITF Antibody is added and binds to ITF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ITF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine ITF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine ITF antibody. ITF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine ITF Antibody is added and binds to ITF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ITF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine ITF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
melanoma-associated antigen D4 isoform 1
NCBI Official Synonym Full Names
MAGE family member D4
NCBI Official Symbol
MAGED4
NCBI Official Synonym Symbols
MAGE1; MAGEE1; MAGE-D4; MAGE-E1
NCBI Protein Information
melanoma-associated antigen D4
UniProt Protein Name
Melanoma-associated antigen D4
Protein Family
UniProt Gene Name
MAGED4
UniProt Synonym Gene Names
KIAA1859; MAGED4A; MAGEE1
UniProt Entry Name
MAGD4_HUMAN
Uniprot Description
MAGE-D4B: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: Xp11
Research Articles on MAGED4
Similar Products
Product Notes
The Canine MAGED4 maged4 (Catalog #AAA1603428) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603428 ELISA Kit recognizes Canine MAGED4. It is sometimes possible for the material contained within the vial of "Melanoma-associated Antigen D4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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