EIF4G recombinant protein

Recombinant Human EIF4G protein, N- His&Trx Tag

Cat. Num. AAA157440

• Gene Names: EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EIF4G; Recombinant Human EIF4G protein; N- His&Trx Tag; EIF4F; EIF4GI; EIF4G recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from 50 mM HEPES, pH7.2, 100 mM KCl containing 2 mM dithiothreitol, 0.5 mM EDTA, 5% Trehalose, 5% Mannose.

• Source: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
• Construction: A DNA sequence encoding the human EIF4G1 (Glu557~Lys646) was fused with the N-terminal His&Trx Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise

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Product Categories/Family for EIF4G recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 302699237
• NCBI GeneID: 1981
• NCBI Accession #: NP_886553.3
• NCBI GenBank Nucleotide #: NM_182917.4
• UniProt Accession #: Q04637; O43177; O95066; Q5HYG0; Q6ZN21; Q8N102; D3DNT4; D3DNT5
• Molecular Weight: 175,491 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4 gamma 1 isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4 gamma, 1
• NCBI Official Symbol: EIF4G1
• NCBI Official Synonym Symbols: P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
• NCBI Protein Information: eukaryotic translation initiation factor 4 gamma 1; EIF4-gamma; eIF-4-gamma 1; eucaryotic translation initiation factor 4G
• UniProt Protein Name: Eukaryotic translation initiation factor 4 gamma 1
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4G1
• UniProt Synonym Gene Names: EIF4F; EIF4G; EIF4GI; eIF-4-gamma 1; eIF-4G 1; eIF-4G1
• UniProt Entry Name: IF4G1_HUMAN

NCBI Description

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]

Uniprot Description

eIF4G: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with CIRBP and MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis. Interacts with RBM4. Belongs to the eIF4G family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; insulin receptor signaling pathway; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay