VAV1 recombinant protein

Recombinant Human VAV1 protein

Cat. Num. AAA1562737

• Gene Names: VAV1; VAV
• Purity: >90% as determined by SDS-PAGE
• Synonyms: VAV1; Recombinant Human VAV1 protein; Proto-oncogene vav; VAV1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human VAV1 (Leu426-Arg696) was fused with GST tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for VAV1 recombinant protein

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Product Categories/Family for VAV1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 7108367
• NCBI GeneID: 7409
• NCBI Accession #: NP_005419.2
• NCBI GenBank Nucleotide #: NM_005428.3
• UniProt Accession #: P15498; Q15860
• Molecular Weight: 98,314 Da
• NCBI Official Full Name: proto-oncogene vav isoform 1
• NCBI Official Synonym Full Names: vav 1 guanine nucleotide exchange factor
• NCBI Official Symbol: VAV1
• NCBI Official Synonym Symbols: VAV
• NCBI Protein Information: proto-oncogene vav; vav 1 oncogene
• UniProt Protein Name: Proto-oncogene vav
• Protein Family: Proto-oncogene
• UniProt Gene Name: VAV1
• UniProt Synonym Gene Names: VAV
• UniProt Entry Name: VAV_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Subunit structure: May interact with CCPG1

By similarity. Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK

By similarity. Interacts with THEMIS2. Interacts with HCK

By similarity. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54

By similarity. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20

Tissue specificity: Widely expressed in hematopoietic cells but not in other cell types.

Domain: The DH domain is involved in interaction with CCPG1

By similarity.

Post-translational modification: Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS)

By similarity. Phosphorylated by FYN. Ref.12

Miscellaneous: 'Vav' stands for the sixth letter of the Hebrew alphabet.

Sequence similarities: Contains 1 CH (calponin-homology) domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 SH2 domain.Contains 2 SH3 domains.

Sequence caution: The sequence CAA34383.1 differs from that shown. Reason: Frameshift at positions 322 and 355.