DHCR24 recombinant protein

Recombinant Human DHCR24 protein

Cat. Num. AAA1560985

• Gene Names: DHCR24; DCE; SELADIN1; Nbla03646; seladin-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: DHCR24; Recombinant Human DHCR24 protein; Delta(24)-sterol reductase; 24-dehydrocholesterol reductase; 3-beta-hydroxysterol Delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1; KIAA0018; DHCR24 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human DHCR24 (Arg53-His516) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for DHCR24 recombinant protein

Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis.In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.

Product Categories/Family for DHCR24 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 20141421
• NCBI GeneID: 1718
• UniProt Accession #: Q15392; Q9HBA8; B7Z817; D3DQ51
• Molecular Weight: 516
• NCBI Official Full Name: Delta(24)-sterol reductase
• NCBI Official Synonym Full Names: 24-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR24
• NCBI Official Synonym Symbols: DCE; SELADIN1; Nbla03646; seladin-1
• NCBI Protein Information: delta(24)-sterol reductase; delta(24)-sterol reductase; seladin 1; diminuto/dwarf1 homolog; selective AD indicator 1; desmosterol-to-cholesterol enzyme; 3 beta-hydroxysterol delta 24-reductase; 3-beta-hydroxysterol delta-24-reductase
• UniProt Protein Name: Delta(24)-sterol reductase
• Protein Family: Delta(24)-sterol reductase
• UniProt Gene Name: DHCR24
• UniProt Synonym Gene Names: KIAA0018
• UniProt Entry Name: DHC24_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.

Protein type: Oxidoreductase; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; EC 1.3.1.72

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; cytosol; nucleus

Molecular Function: delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; FAD binding; peptide antigen binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity

Biological Process: skin development; tissue development; negative regulation of cell proliferation; amyloid precursor protein catabolic process; protein localization; apoptosis; Ras protein signal transduction; male genitalia development; response to hormone stimulus; negative regulation of caspase activity; response to oxidative stress; cell cycle arrest; cholesterol biosynthetic process; plasminogen activation; negative regulation of apoptosis

Disease: Desmosterolosis