Rabbit anti-Human IL-11 Polyclonal Antibody | anti-IL-11 antibody

IL-11 Antibody

Cat. Num. AAA154367

• Gene Names: IL11; AGIF; IL-11
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: IL-11; Polyclonal Antibody; IL-11 Antibody; Interleukin-11; Adipogenesis inhibitory factor; AGIF; anti-IL-11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL-11 antibody is human specific. At least two isoforms of IL-11 are known to exist; this antibody will only detect the longest isoform of IL-11.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 199

• Applicable Applications for anti-IL-11 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: IL-11 antibody can be used for the detection of IL-11 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry at 10 mug/ml.
• Immunogen: IL-11 antibody was raised against a 16 amino acid peptide from near the amino terminus of human IL-11.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: HeLa Cell Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of IL-11 in Hela Cell lysate with IL-11 antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of IL-11 in HeLa cells with IL-11 antibody at 10 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IL-11 in HeLa cells with IL-11 antibody at 20 μg/ml.Red: IL-11 Antibody (8453)Blue: DAPI staining)

Related Product Information for anti-IL-11 antibody

Interleukin 11 is a pleiotropic cytokine that has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6 (1). It directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (2). IL-11 promotes the proliferation of hepatocytes in response to liver damage. IL-11 binds to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 (3). Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelo-suppressive chemotherapy (4).

Product Categories/Family for anti-IL-11 antibody

Stem Cell; Immunology

References

Paul SR, Bennett F, Calvetti JA. Molecular cloning of a cDNA encoding interleukin-11, astromal cell-derived lymphopoietic and hematopoietic cytokine. Proc. Natl. Acad. Sci. USA 1990; 87:7512-6. Yang YC and Yin T. Interleukin (IL)-11 mediated signal transduction. Ann. N.Y. Acad. Sci. 1995; 762:31-40. Dimitriadis E, Robb L, Salamonsen LA. Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells. Mol. Hum. Reprod. 2002; 8:636-43. Andersson By U, Tani E, Andersson U. Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis. J. Pediatr. Hematol. Oncol. 2004; 26:706-11.

NCBI and Uniprot Product Information

• NCBI GI #: 10834994
• NCBI GeneID: 3589
• NCBI Accession #: NP_000632
• NCBI GenBank Nucleotide #: NM_000641.3
• UniProt Accession #: P20809; Q96EB4; B4DQV5
• Molecular Weight: Predicted: 20 kDa; Observed: 19 kDa
• NCBI Official Full Name: interleukin-11 isoform 1
• NCBI Official Synonym Full Names: interleukin 11
• NCBI Official Symbol: IL11
• NCBI Official Synonym Symbols: AGIF; IL-11
• NCBI Protein Information: interleukin-11
• UniProt Protein Name: Interleukin-11
• UniProt Gene Name: IL11
• UniProt Synonym Gene Names: AGIF
• UniProt Entry Name: IL11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily.

Protein type: Cytokine; Motility/polarity/chemotaxis; Cell cycle regulation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; interleukin-11 receptor binding; protein binding

Biological Process: B cell differentiation; cell-cell signaling; fat cell differentiation; megakaryocyte differentiation; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter