Hemagglutinin Recombinant Protein | HA recombinant protein

Hemagglutinin Recombinant Protein

Cat. Num. AAA154165

• Applications: ELISA, Western Blot
• Purity: ~95%
• Synonyms: Hemagglutinin; Hemagglutinin Recombinant Protein; H5N1 Hemagglutinin HA1; H5N1 Hemagglutinin; H5N1 HA1; HA1; HA recombinant protein

• Host: E Coli
• Purity/Purification: ~95%
• Form/Format: Liquid; 1X PBS containing 0.1% SDS and 0.02% NaN3
• Sequence: aa. 17-338

• Applicable Applications for HA recombinant protein: ELISA (EIA), Western Blot (WB)
• Fusion Tag: Fusion Partner: His-tag and strepll-tag at N-Terminus
• Sequence: aa. 17-338
• Species: Influenza A virus (A/chicken/Jilin/9/2004(H5N1))
• Preparation and Storage: Ships on dry ice. Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

SDS-PAGE

(SDS-PAGE analysis of Avian Influenza (H5) Hemagglutinin subunit HA1 on Coomassie Blue-stained 4-20% gradient gel.)

Related Product Information for HA recombinant protein

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals, but it is in birds that all subtypes can be found (2). These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with host cell surface proteins containing oligosaccharides with terminal sialyl residues. Its extracellular region has two domains (HA1 and HA2); HA1 is cleaved from the main hemagglutinin protein by the host immune system. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3). This more recent virulent strain of H5N1 is now seen in Africa and Europe, as well as in Southeast Asia. There is some evidence of human to human spread of this virus, but it is thought that the efficiency of this type of transmission is low (4). Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability (5). This HA1 recombinant protein is recognized by several hemagglutinin antibodies.

NCBI and Uniprot Product Information

• NCBI GI #: 50365729
• NCBI GeneID: 3654620
• UniProt Accession #: Q692M2; Q9Q0U6
• Molecular Weight: 39kDa (calculated)
• NCBI Official Full Name: hemagglutinin
• NCBI Official Symbol: HA
• NCBI Protein Information: hemagglutinin; HA1; HA2
• UniProt Protein Name: Hemagglutinin
• Protein Family: Hemagglutinin
• UniProt Gene Name: HA
• UniProt Entry Name: Q692M2_9INFA

Uniprot Description

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.