MAP1LC3C blocking peptide

MAP1LC3C Peptide

Cat. Num. AAA153795

• Gene Names: MAP1LC3C; LC3C; ATG8J
• Applications: Blocking (BL)
• Synonyms: MAP1LC3C; MAP1LC3C Peptide; LC3C; ATG8JAutophagy-related protein LC3 C; microtubule-associated protein 1 light chain 3 gamma; MAP1LC3C blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 147

• Applicable Applications for MAP1LC3C blocking peptide: Blocking (BL)
• Application Notes: The peptide is used for blocking the activity of MAP1LC3C antibody. It usually blocks the antibody activity completely by incubating the peptide with equal volume of antibody for 30 min at 37 degree C.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 18 amino acids near the carboxy terminus of human MAP1LC3C.
• Preparation and Storage: Store MAP1LC3C peptide at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of MAP1LC3C in human brain tissue lysate with MAP1LC3C antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 51972260
• NCBI GeneID: 440738
• NCBI Accession #: NP_001004343
• NCBI GenBank Nucleotide #: NM_001004343.2
• UniProt Accession #: Q9BXW4; A0PJY8; A2RUP0
• Molecular Weight: 16,852 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3C
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 gamma
• NCBI Official Symbol: MAP1LC3C
• NCBI Official Synonym Symbols: LC3C; ATG8J
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B LC3 C; LC3-like protein 2; MAP1A/MAP1B light chain 3 C; autophagy-related protein LC3 C; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; autophagy-related ubiquitin-like modifier LC3 C
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3C
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3C
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 C
• UniProt Entry Name: MLP3C_HUMAN

NCBI Description

Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]

Uniprot Description

LC3C: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes.

Protein type: Ubiquitin-like modifier; Autophagy; Vesicle; Microtubule-binding

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: microtubule; extrinsic to membrane; endomembrane system; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation