Typical Testing Data/Standard Curve (for reference only)
Regenerating islet-derived protein 3-gamma ELISA Kit | REG3G elisa kit
Rat Regenerating islet-derived protein 3-gamma ELISA Kit
Gene Names
REG3G; PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
Reactivity
Rat
Synonyms
Regenerating islet-derived protein 3-gamma; Rat Regenerating islet-derived protein 3-gamma ELISA Kit; REG3G elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of REG3G. No significant cross-reactivity or interference between REG3G and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between REG3G and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for REG3G elisa kit
Intended Uses: This REG3G ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat REG3G. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: REG3G ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-REG3G antibody and an REG3G-HRP conjugate. The assay sample and buffer are incubated together with REG3G-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the REG3G concentration since REG3G from samples and REG3G-HRP conjugate compete for the anti-REG3G antibody binding site. Since the number of sites is limited, as more sites are occupied by REG3G from the sample, fewer sites are left to bind REG3G-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The REG3G concentration in each sample is interpolated from this standard curve.
Principle of the Assay: REG3G ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-REG3G antibody and an REG3G-HRP conjugate. The assay sample and buffer are incubated together with REG3G-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the REG3G concentration since REG3G from samples and REG3G-HRP conjugate compete for the anti-REG3G antibody binding site. Since the number of sites is limited, as more sites are occupied by REG3G from the sample, fewer sites are left to bind REG3G-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The REG3G concentration in each sample is interpolated from this standard curve.
Product Categories/Family for REG3G elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,409 Da
NCBI Official Full Name
regenerating islet-derived protein 3-gamma isoform 1
NCBI Official Synonym Full Names
regenerating islet-derived 3 gamma
NCBI Official Symbol
REG3G
NCBI Official Synonym Symbols
PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; regenerating gene III; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; regenerating islet-derived protein III-gamma
UniProt Protein Name
Regenerating islet-derived protein 3-gamma
Protein Family
UniProt Gene Name
REG3G
UniProt Synonym Gene Names
PAP1B; UNQ429/PRO162; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma
UniProt Entry Name
REG3G_HUMAN
NCBI Description
This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antibacterial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]
Uniprot Description
REG3G: Might be a stress protein involved in the control of bacterial proliferation.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: cytoplasm; extracellular region
Molecular Function: carbohydrate binding
Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response
Research Articles on REG3G
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Product Notes
The Rat REG3G reg3g (Catalog #AAA756017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA756017 ELISA Kit recognizes Rat REG3G. It is sometimes possible for the material contained within the vial of "Regenerating islet-derived protein 3-gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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