Rabbit SPIB Polyclonal Antibody | anti-SPIB antibody

SPIB Antibody

Cat. Num. AAA153544

• Gene Names: SPIB; SPI-B
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: SPIB antibody is affinity chromatography purified via peptide column.
• Synonyms: SPIB; Polyclonal Antibody; SPIB Antibody; Spi-B transcription factor; SPI-B; Spi-B transcription factor (Spi-1/PU.1 related); anti-SPIB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.
• Purity/Purification: SPIB antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 262

• Applicable Applications for anti-SPIB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 mug/ml.
• Conjugate: Unconjugated
• Immunogen: SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB.
• Buffer: SPIB antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SPIB antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of SPIB in human prostate tissue lysate with SPIB antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of SPIB in human prostate tissue with SPIB Antibody at 20 μg/mL.)

Related Product Information for anti-SPIB antibody

SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1). SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3). It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN)-producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4).

NCBI and Uniprot Product Information

• NCBI GI #: 61888836
• NCBI GeneID: 6689
• NCBI Accession #: NP_003112
• NCBI GenBank Nucleotide #: NM_003121.4
• UniProt Accession #: Q01892; Q15359; A8K9C9; B4DUG6
• Molecular Weight: Predicted: 19; 25; 29 kDa; ; Observed: 20 kDa
• NCBI Official Full Name: transcription factor Spi-B isoform 1
• NCBI Official Synonym Full Names: Spi-B transcription factor (Spi-1/PU.1 related)
• NCBI Official Symbol: SPIB
• NCBI Official Synonym Symbols: SPI-B
• NCBI Protein Information: transcription factor Spi-B
• UniProt Protein Name: Transcription factor Spi-B
• Protein Family: Transcription factor
• UniProt Gene Name: SPIB
• UniProt Entry Name: SPIB_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPIB: Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell differentiation