Rabbit anti-Human, Mouse KANK1 Polyclonal Antibody | anti-KANK1 antibody

KANK1 Antibody

Cat. Num. AAA153365

• Gene Names: KANK1; KANK; CPSQ2; ANKRD15
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: KANK1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KANK1; Polyclonal Antibody; KANK1 Antibody; KANK; CPSQ2; ANKRD15; KIAA0172; KN motif and ankyrin repeat domain-containing protein 1; Ankyrin repeat domain-containing protein 15; KN motif and ankyrin repeat domains 1; anti-KANK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Two alternatively spliced transcript variants encoding different isoforms have been identified. The lower molecular weight band seen in the immunoblot is thought to be non-specific.
• Purity/Purification: KANK1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1352

• Applicable Applications for anti-KANK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: KANK1 Antibody can be used for detection of KANK1 by Western blot at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: KANK1 antibody was raised against a 19 amino acid peptide near the amino terminus of human KANK1.
• Buffer: KANK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KANK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of KANK1 in 3T3 cell lysate with KANK1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of KANK1 in human kidney tissue with KANK1 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of KANK1 in human kidney tissue with KANK1 antibody at 20 μg/ml.)

Related Product Information for anti-KANK1 antibody

KANK1 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK1 (KN motif and ankyrin repeat domain-containing protein 1), also known as ANKRD15, is a 1,352 amino acid protein that contains at least 12 exons and 5 ANK repeats. It binds to beta-catenin and regulates its subcellular distribution. KANK1 is ubiquitously expressed and localizes to cytoplasm. It may function as a tumor suppressor for renal cell carcinoma. Mutations in this gene cause cerebral palsy spastic quadriplegic type 2, a central nervous system development disorder.

NCBI and Uniprot Product Information

• NCBI GI #: 64464726
• NCBI GeneID: 23189
• NCBI Accession #: NP_055973
• NCBI GenBank Nucleotide #: NM_015158.3
• UniProt Accession #: Q14678; Q5W0W0; Q8IY65; Q8WX74; A2A2W8; D3DRH3
• Molecular Weight: Predicted: 149 kDa; ; Observed: 150 kDa
• NCBI Official Full Name: KN motif and ankyrin repeat domain-containing protein 1 isoform a
• NCBI Official Synonym Full Names: KN motif and ankyrin repeat domains 1
• NCBI Official Symbol: KANK1
• NCBI Official Synonym Symbols: KANK; CPSQ2; ANKRD15
• NCBI Protein Information: KN motif and ankyrin repeat domain-containing protein 1; kidney ankyrin repeat-containing protein; ankyrin repeat domain-containing protein 15
• UniProt Protein Name: KN motif and ankyrin repeat domain-containing protein 1
• Protein Family: KN motif and ankyrin repeat domain-containing protein
• UniProt Gene Name: KANK1
• UniProt Synonym Gene Names: ANKRD15; KANK; KIAA0172
• UniProt Entry Name: KANK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Kank family of proteins, which contain multiple ankyrin repeat domains. This family member functions in cytoskeleton formation by regulating actin polymerization. This gene is a candidate tumor suppressor for renal cell carcinoma. Mutations in this gene cause cerebral palsy spastic quadriplegic type 2, a central nervous system development disorder. A t(5;9) translocation results in fusion of the platelet-derived growth factor receptor beta gene (PDGFRB) on chromosome 5 with this gene in a myeloproliferative neoplasm featuring severe thrombocythemia. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2014]

Uniprot Description

ANKRD15: Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma. Defects in KANK1 are the cause of cerebral palsy spastic quadriplegic type 2 (CPSQ2). A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest congenital hypotonia evolving over the first year to spastic quadriplegia with accompanying transient nystagmus and varying degrees of mental retardation. Neuroimaging shows brain atrophy and ventriculomegaly. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; beta-catenin binding

Biological Process: negative regulation of Rho protein signal transduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of actin filament polymerization; negative regulation of insulin receptor signaling pathway; positive regulation of Wnt receptor signaling pathway; negative regulation of cell migration

Disease: Cerebral Palsy, Spastic Quadriplegic, 2