SIRT2 blocking peptide

SIRT2 Peptide

Cat. Num. AAA152962

• Gene Names: SIRT2; SIR2; SIR2L; SIR2L2
• Applications: Blocking (BL)
• Synonyms: SIRT2; SIRT2 Peptide; SIR2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae); SIRT2 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 389

• Applicable Applications for SIRT2 blocking peptide: Blocking (BL)
• Application Notes: SIRT2 peptide is used for blocking the activity of Sirt2 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 19 amino acids near the carboxy terminus of human Sirt2.
• Preparation and Storage: Store SIRT2 peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 13775600
• NCBI GeneID: 22933
• NCBI Accession #: NP_036369
• NCBI GenBank Nucleotide #: NM_012237.3
• UniProt Accession #: Q8IXJ6; O95889; Q924Y7; Q9P0G8; Q9UNT0; Q9Y6E9; A8K3V1; B2RB45; U5TP13
• Molecular Weight: 35,654 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-2 isoform 1
• NCBI Official Synonym Full Names: sirtuin 2
• NCBI Official Symbol: SIRT2
• NCBI Official Synonym Symbols: SIR2; SIR2L; SIR2L2
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-2; sirtuin type 2; SIR2-like protein 2; sir2-related protein type 2; silent information regulator 2; regulatory protein SIR2 homolog 2; NAD-dependent deacetylase sirtuin-2
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-2
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT2
• UniProt Synonym Gene Names: SIR2L; SIR2L2
• UniProt Entry Name: SIR2_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

SIRT2: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: centrosome; microtubule; lateral loop; paranode region of axon; perikaryon; chromosome; nuclear heterochromatin; cytosol; centriole; chromatin silencing complex; growth cone; perinuclear region of cytoplasm; cytoplasm; plasma membrane; spindle; midbody; nucleus; myelin sheath

Molecular Function: histone acetyltransferase binding; protein binding; ubiquitin binding; tubulin deacetylase activity; zinc ion binding; NAD-dependent histone deacetylase activity; histone deacetylase binding; protein deacetylase activity; beta-tubulin binding; chromatin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding; NAD+ ADP-ribosyltransferase activity

Biological Process: chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of autophagy; phosphoinositide 3-kinase cascade; regulation of myelination; regulation of cell cycle; chromatin silencing; positive regulation of attachment of spindle microtubules to kinetochore; response to redox state; gene silencing; negative regulation of transcription from RNA polymerase II promoter; chromatin silencing at telomere; negative regulation of cell proliferation; substantia nigra development; meiotic cell cycle; protein amino acid deacetylation; cellular lipid catabolic process; protein kinase B signaling cascade; positive regulation of DNA binding; mitosis; positive regulation of meiosis; transcription, DNA-dependent; regulation of phosphorylation; regulation of exit from mitosis; histone deacetylation; hepatocyte growth factor receptor signaling pathway; negative regulation of striated muscle development; negative regulation of fat cell differentiation; myelination in the peripheral nervous system; protein amino acid ADP-ribosylation; cell division; positive regulation of cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; autophagy; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process