XBP-1 Blocking Peptide | XBP1 blocking peptide

XBP-1 Peptide

Cat. Num. AAA152902

• Gene Names: XBP1; XBP2; TREB5; XBP-1
• Applications: Blocking (BL)
• Synonyms: XBP-1; XBP-1 Peptide; XBP2; TREB5; X-box-binding protein 1; Tax-responsive element-binding protein 5; X-box binding protein 1; XBP1 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 261

• Applicable Applications for XBP1 blocking peptide: Blocking (BL)
• Application Notes: XBP-1 peptide is used for blocking the activity of XBP-1 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 17 amino acids near the carboxy terminus of human XBP-1.
• Preparation and Storage: Store XBP-1 peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 60416406
• NCBI GeneID: 7494
• UniProt Accession #: P17861; Q8WYK6; Q969P1; Q96BD7
• Molecular Weight: 40,148 Da
• NCBI Official Full Name: X-box-binding protein 1
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: XBP1
• NCBI Official Synonym Symbols: XBP2; TREB5; XBP-1
• NCBI Protein Information: X-box-binding protein 1; tax-responsive element-binding protein 5
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: XBP1
• UniProt Synonym Gene Names: TREB5; XBP2; XBP-1
• UniProt Entry Name: XBP1_HUMAN

NCBI Description

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q12.1|22q12

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protease binding; DNA binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; estrogen receptor binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; phosphoinositide 3-kinase cascade; muscle development; apoptosis; exocrine pancreas development; positive regulation of transcription of target genes involved in unfolded protein response; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; cellular response to glucose starvation; protein transport; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; cell growth; response to electrical stimulus; positive regulation of TOR signaling pathway; fatty acid biosynthetic process; positive regulation of autophagy; response to drug; positive regulation of histone methylation; protein destabilization; unfolded protein response; organelle organization and biogenesis; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; positive regulation of immunoglobulin production; cholesterol homeostasis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; positive regulation of T cell differentiation; endothelial cell proliferation; fatty acid homeostasis; positive regulation of B cell differentiation; autophagy; positive regulation of fat cell differentiation; neuron development; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; sterol homeostasis; negative regulation of apoptosis

Disease: Major Affective Disorder 7