Typical Testing Data/Standard Curve (for reference only)
Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 2 (AGAP2) ELISA Kit | AGAP2 elisa kit
Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 2 (AGAP2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AGAP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AGAP2 antibody and an AGAP2-HRP conjugate. The assay sample and buffer are incubated together with AGAP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGAP2 concentration since AGAP2 from samples and AGAP2-HRP conjugate compete for the anti-AGAP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGAP2 from the sample, fewer sites are left to bind AGAP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGAP2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
CENTG1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.
Protein type: Tumor suppressor; GAPs, ARF; GAPs; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q14.1
Cellular Component: membrane; mitochondrion; nucleolus; nucleus
Molecular Function: protein binding; GTP binding; zinc ion binding
Biological Process: axon guidance; protein transport; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; positive regulation of GTPase activity
Research Articles on AGAP2
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Product Notes
The Human AGAP2 agap2 (Catalog #AAA7200291) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7200291 ELISA Kit recognizes Human AGAP2. It is sometimes possible for the material contained within the vial of "Arf GAP with GTPase, ANK repeat and PH domain containing protein 2 (AGAP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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