DAD1 blocking peptide

DAD1 Peptide

Cat. Num. AAA152867

• Gene Names: DAD1; OST2
• Applications: Blocking (BL)
• Synonyms: DAD1; DAD1 Peptide; OST2; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Defender against cell death 1; Oligosaccharyl transferase subunit DAD1; defender against cell death 1; DAD1 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 113

• Applicable Applications for DAD1 blocking peptide: Blocking (BL)
• Application Notes: DAD1 peptide is used for blocking the activity of DAD1 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 14 amino acids near the amino terminus of human DAD1.
• Preparation and Storage: Store DAD1 peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 14602573
• NCBI GeneID: 1603
• UniProt Accession #: P61803; O08552; O70364; P46966; P46968; Q6FGA3; Q96GB7; D3DS25
• Molecular Weight: 12,497 Da
• NCBI Official Full Name: Defender against cell death 1
• NCBI Official Synonym Full Names: defender against cell death 1
• NCBI Official Symbol: DAD1
• NCBI Official Synonym Symbols: OST2
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic)
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• Protein Family: DASH complex
• UniProt Gene Name: DAD1
• UniProt Synonym Gene Names: Oligosaccharyl transferase subunit DAD1; DAD-1
• UniProt Entry Name: DAD1_HUMAN

NCBI Description

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]

Uniprot Description

DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.

Protein type: Endoplasmic reticulum; EC 2.4.99.18; Membrane protein, integral; Transferase; Membrane protein, multi-pass; Glycan Metabolism - N-glycan biosynthesis; Apoptosis

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complex; integral to membrane

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity

Biological Process: response to drug; blastocyst development; cellular protein metabolic process; apoptosis; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to nutrient; negative regulation of apoptosis