RAP80 Blocking Peptide | UIMC1 blocking peptide

RAP80 Peptide

Cat. Num. AAA152851

• Gene Names: UIMC1; RAP80; X2HRIP110
• Applications: Blocking (BL)
• Synonyms: RAP80; RAP80 Peptide; X2HRIP110; RXRIP110; BRCA1-A complex subunit RAP80; Receptor-associated protein 80; ubiquitin interaction motif containing 1; UIMC1 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 719

• Applicable Applications for UIMC1 blocking peptide: Blocking (BL)
• Application Notes: RAP80 peptide is used for blocking the activity of RAP80 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 17 amino acids near the carboxy terminus of human RAP80.
• Preparation and Storage: Store RAP80 peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 119605449
• NCBI GeneID: 51720
• UniProt Accession #: Q96RL1; Q5XKQ1; Q7Z3W7; Q8N5B9; Q9BZR1; Q9BZR5; Q9UHX7; A8MSA1; B3KMZ1; B4E3N2
• Molecular Weight: 17,412 Da
• NCBI Official Full Name: receptor associated protein 80, isoform CRA_c
• NCBI Official Synonym Full Names: ubiquitin interaction motif containing 1
• NCBI Official Symbol: UIMC1
• NCBI Official Synonym Symbols: RAP80; X2HRIP110
• NCBI Protein Information: BRCA1-A complex subunit RAP80; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1
• UniProt Protein Name: BRCA1-A complex subunit RAP80
• Protein Family: BRCA1-A complex
• UniProt Gene Name: UIMC1
• UniProt Synonym Gene Names: RAP80; RXRIP110
• UniProt Entry Name: UIMC1_HUMAN

Uniprot Description

RAP80: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Interacts with TSP57. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Belongs to the RAP80 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: nucleus

Molecular Function: retinoid X receptor binding; protein binding; histone binding

Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; double-strand break repair; response to ionizing radiation; G2/M transition DNA damage checkpoint; negative regulation of transcription, DNA-dependent