Western Blot (WB)
(Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.)
Rabbit anti-Human, Mouse Hrk Polyclonal Antibody | anti-HRK antibody
Hrk Antibody
Gene Names
HRK; DP5; HARAKIRI
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Hrk Antibody is affinity chromatography purified via peptide column.
Synonyms
Hrk; Polyclonal Antibody; Hrk Antibody; DP5; HARAKIRI; BID3; Activator of apoptosis harakiri; BH3-interacting domain-containing protein 3; harakiri; BCL2 interacting protein (contains only BH3 domain); anti-HRK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Hrk Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
91
Applicable Applications for anti-HRK antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 mug/mL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
Conjugate
Unconjugated
Immunogen
HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk.
Buffer
Hrk Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Hrk antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.)
Western Blot (WB)
(Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.)
Related Product Information for anti-HRK antibody
Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,884 Da
NCBI Official Full Name
activator of apoptosis harakiri
NCBI Official Synonym Full Names
harakiri, BCL2 interacting protein
NCBI Official Symbol
HRK
NCBI Official Synonym Symbols
DP5; HARAKIRI
NCBI Protein Information
activator of apoptosis harakiri; death protein 5; BCL2-interacting protein; activator of apoptosis Hrk; neuronal death protein DP5; BH3-interacting domain-containing protein 3; harakiri, BCL2 interacting protein (contains only BH3 domain)
UniProt Protein Name
Activator of apoptosis harakiri
Protein Family
UniProt Gene Name
HRK
UniProt Synonym Gene Names
BID3
UniProt Entry Name
HRK_HUMAN
NCBI Description
This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
Uniprot Description
HRK: Promotes apoptosis.
Protein type: Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 12q24.22
Cellular Component: mitochondrion; integral to membrane
Molecular Function: protein binding
Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization
Research Articles on HRK
Similar Products
Product Notes
The HRK hrk (Catalog #AAA151175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hrk Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hrk can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 mug/mL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa. Researchers should empirically determine the suitability of the HRK hrk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hrk, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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