Western Blot (WB)
(Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at 0.5 μg/mL.)
Rabbit anti-Human, Mouse ZFP281 Polyclonal Antibody | anti-ZNF281 antibody
ZFP281 Antibody
Gene Names
ZNF281; ZBP-99; ZNP-99
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence
Purity
ZFP281 Antibody is affinity chromatography purified via peptide column.
Synonyms
ZFP281; Polyclonal Antibody; ZFP281 Antibody; ZBP-99; ZNP-99; GZP1; ZBP99; Zinc finger protein 281; GC-box-binding zinc finger protein 1; zinc finger protein 281; anti-ZNF281 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
ZFP281 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
895
Applicable Applications for anti-ZNF281 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.25 - 0.5 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ZFP281 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281.
Buffer
ZFP281 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ZFP281 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at 0.5 μg/mL.)
Western Blot (WB)
(Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at 0.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 μg/mL.)
Related Product Information for anti-ZNF281 antibody
ZFP281 Antibody: ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,232 Da
NCBI Official Full Name
zinc finger protein 281 isoform 1
NCBI Official Synonym Full Names
zinc finger protein 281
NCBI Official Symbol
ZNF281
NCBI Official Synonym Symbols
ZBP-99; ZNP-99
NCBI Protein Information
zinc finger protein 281; ZNP-99 transcription factor; transcription factor ZBP-99; zinc finger DNA-binding protein 99; GC-box-binding zinc finger protein 1
UniProt Protein Name
Zinc finger protein 281
Protein Family
UniProt Gene Name
ZNF281
UniProt Synonym Gene Names
GZP1; ZBP99
UniProt Entry Name
ZN281_HUMAN
Uniprot Description
ZNF281: Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity; transcription corepressor activity
Biological Process: embryonic body morphogenesis; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Research Articles on ZNF281
Similar Products
Product Notes
The ZNF281 znf281 (Catalog #AAA151071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZFP281 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZFP281 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.25 - 0.5 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ZNF281 znf281 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFP281, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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