Western Blot (WB)
(Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.)
Rabbit ZFP219 Polyclonal Antibody | anti-ZNF219 antibody
ZFP219 Antibody
Gene Names
ZNF219; ZFP219
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ZFP219 Antibody is affinity chromatography purified via peptide column.
Synonyms
ZFP219; Polyclonal Antibody; ZFP219 Antibody; Zinc finger protein 219; zinc finger protein 219; anti-ZNF219 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.
Purity/Purification
ZFP219 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
722
Applicable Applications for anti-ZNF219 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219.
Buffer
ZFP219 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ZFP219 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.)
Related Product Information for anti-ZNF219 antibody
ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,877 Da
NCBI Official Full Name
zinc finger protein 219
NCBI Official Synonym Full Names
zinc finger protein 219
NCBI Official Symbol
ZNF219
NCBI Official Synonym Symbols
ZFP219
NCBI Protein Information
zinc finger protein 219
UniProt Protein Name
Zinc finger protein 219
Protein Family
UniProt Gene Name
ZNF219
UniProt Entry Name
ZN219_HUMAN
Uniprot Description
ZNF219: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 14q11
Cellular Component: integral to membrane; nucleus
Molecular Function: protein binding; DNA binding; metal ion binding; histamine receptor activity; transcription factor activity
Biological Process: G-protein coupled receptor protein signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of neurotransmitter levels; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Research Articles on ZNF219
Similar Products
Product Notes
The ZNF219 znf219 (Catalog #AAA150420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZFP219 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZFP219 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ZNF219 znf219 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFP219, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
