Rabbit Emerin Polyclonal Antibody | anti-EMD antibody

Emerin Antibody

Cat. Num. AAA150246

• Gene Names: EMD; STA; EDMD; LEMD5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Emerin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Emerin; Polyclonal Antibody; Emerin Antibody; STA; EDMD; LEMD5; emerin; anti-EMD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Emerin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 254

• Applicable Applications for anti-EMD antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Emerin antibody can be used for detection of Emerin by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Emerin antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Emerin.
• Buffer: Emerin Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Emerin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Emerin in human skeletal muscle tissue lysate with Emerin antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of emerin in human skeletal muscle tissue with emerin antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Emerin in Human Skeletal Muscle cells with Emerin antibody at 5 μg/mL.)

Related Product Information for anti-EMD antibody

Emerin Antibody: Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family that includes proteins such as LAP2 and MAN1. Each family member, including Emerin, has an ~40 amino acid LEM-domains that binds barrier-to-autointegration (BANF1), a conserved chromatin protein that also serves as a host cell component of retroviral integration complexes, including that of HIV. Emerin is anchored at the inner membrane of the nuclear envelope where it binds to nuclear intermediate filaments that are formed by lamin proteins. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4557553
• NCBI GeneID: 2010
• NCBI Accession #: NP_000108
• NCBI GenBank Nucleotide #: NM_000117.2
• UniProt Accession #: P50402; Q6FI02
• Molecular Weight: 28,994 Da
• NCBI Official Full Name: emerin
• NCBI Official Synonym Full Names: emerin
• NCBI Official Symbol: EMD
• NCBI Official Synonym Symbols: STA; EDMD; LEMD5
• NCBI Protein Information: emerin; LEM domain containing 5
• UniProt Protein Name: Emerin
• Protein Family: Emerin
• UniProt Gene Name: EMD
• UniProt Synonym Gene Names: EDMD; STA
• UniProt Entry Name: EMD_HUMAN

NCBI Description

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]

Uniprot Description

emerin: a single pass membrane protein that spans the inner nuclear membrane. A serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It interacts with lamins A and C, and mediates membrane anchorage to the cytoskeleton. Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through an exportin 1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Colocalized with BAF at the central region of the assembling nuclear rim, near spindle-attachment sites. Hyper-phosphorylated on tyrosine in cells overexpressing HER2. Directly phosphorylated by Src and Abl. Phosphorylated by Src at Y59, Y74 and Y95. May function as a downstream effector and signal integrator for tyrosine kinase signaling pathways at the nuclear envelope. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Protein type: Cytoskeletal; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: microtubule; nuclear outer membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear inner membrane; nuclear envelope

Molecular Function: protein binding; beta-tubulin binding; actin binding

Biological Process: positive regulation of protein export from nucleus; mitotic nuclear envelope reassembly; muscle development; muscle contraction; negative regulation of fibroblast proliferation; mitotic nuclear envelope disassembly; mitotic cell cycle

Disease: Emery-dreifuss Muscular Dystrophy 1, X-linked