Rabbit anti-Human, Mouse DDX3 Polyclonal Antibody | anti-DDX3X antibody

DDX3 Antibody

Cat. Num. AAA150245

• Gene Names: DDX3X; DBX; DDX3; HLP2; DDX14; CAP-Rf
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: DDX3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DDX3; Polyclonal Antibody; DDX3 Antibody; DBX; HLP2; DDX14; ATP-dependent RNA helicase DDX3X; DEAD box protein 3; X-chromosomal; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3; X-linked; anti-DDX3X antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDX3 antibody will detect both DDX3 and DBY.
• Purity/Purification: DDX3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 662

• Applicable Applications for anti-DDX3X antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3.
• Buffer: DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DDX3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.)

Related Product Information for anti-DDX3X antibody

DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

NCBI and Uniprot Product Information

• NCBI GI #: 3523150
• NCBI GeneID: 1654
• UniProt Accession #: O00571; O15536; A8K538; B4E3E8
• Molecular Weight: 71,355 Da
• NCBI Official Full Name: DEAD box RNA helicase DDX3
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
• NCBI Official Symbol: DDX3X
• NCBI Official Synonym Symbols: DBX; DDX3; HLP2; DDX14; CAP-Rf
• NCBI Protein Information: ATP-dependent RNA helicase DDX3X; DEAD/H box-3; DEAD box, X isoform; helicase-like protein 2; DEAD box protein 3, X-chromosomal; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• UniProt Protein Name: ATP-dependent RNA helicase DDX3X
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX3X
• UniProt Synonym Gene Names: DBX; DDX3; HLP2
• UniProt Entry Name: DDX3X_HUMAN

NCBI Description

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

DDX3: a multifunctional DEAD box family RNA helicase with diverse cellular functions. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), and are involved in several steps of gene expression, such as transcription, maturation of nuclear and mitochondrial mRNA, mRNA export, translation initiation, and ribosome and spliceosome assembly. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). May function as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. Associates with eIF4F to promote translation of selected mRNAs. Is phosphorylated by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation may cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFN-beta induction. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Regulated by the cell cycle. Maximally expressed in the cytoplasm during G1/S phase and decreased expression during G2/M phase. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Associates with the outer side of nuclear pore complexes (NPC) and mitochondrial outer membranes. Shuttles between the nucleus and the cytoplasm in an exportin1-dependent manner. Associates with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. Belongs to the DEAD box helicase family, DDX3 subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; Helicase; EC 3.6.4.13; Translation initiation; RNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: eukaryotic translation initiation factor 3 complex; mitochondrial outer membrane; stress granule; cytoplasm; nuclear speck; nucleus

Molecular Function: GTPase activity; CTPase activity; RNA binding; ATPase activity; transcription factor binding; poly(A) binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; translation initiation factor binding; eukaryotic initiation factor 4E binding; nucleoside-triphosphatase activity; mRNA 5'-UTR binding; ATP binding; ATP-dependent RNA helicase activity; ribosomal small subunit binding

Biological Process: stress granule assembly; positive regulation of viral genome replication; viral reproduction; positive regulation of translation; transcription, DNA-dependent; positive regulation of apoptosis; response to virus; positive regulation of caspase activity; negative regulation of caspase activity; positive regulation of cell growth; DNA duplex unwinding; chromosome segregation; positive regulation of translational initiation; induction of apoptosis via death domain receptors; RNA secondary structure unwinding; negative regulation of translation; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of protein complex assembly; mature ribosome assembly; negative regulation of apoptosis

Disease: Mental Retardation, X-linked 102