Western Blot (WB)
(Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit JMJD1B Polyclonal Antibody | anti-KDM3B antibody
JMJD1B Antibody
Gene Names
KDM3B; 5qNCA; NET22; C5orf7; JMJD1B
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
JMJD1B Antibody is affinity chromatography purified via peptide column.
Synonyms
JMJD1B; Polyclonal Antibody; JMJD1B Antibody; 5qNCA; NET22; C5orf7; JHDM2B; KIAA1082; Lysine-specific demethylase 3B; JmjC domain-containing histone demethylation protein 2B; lysine (K)-specific demethylase 3B; anti-KDM3B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody will not cross-react with JMJD1A or JMJD1C.
Purity/Purification
JMJD1B Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1761
Applicable Applications for anti-KDM3B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
JMJD1B antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B.
Buffer
JMJD1B Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
JMJD1B antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of JMJD1B in rat liver tissue with JMJD1B antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of JMJD1B in rat liver tissue with JMJD1B antibody at 20 μg/mL.)
Related Product Information for anti-KDM3B antibody
JMJD1B Antibody: The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
84,712 Da
NCBI Official Full Name
jumonji domain containing 1B, isoform CRA_a
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 3B
NCBI Official Symbol
KDM3B
NCBI Official Synonym Symbols
5qNCA; NET22; C5orf7; JMJD1B
NCBI Protein Information
lysine-specific demethylase 3B; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
UniProt Protein Name
Lysine-specific demethylase 3B
Protein Family
UniProt Gene Name
KDM3B
UniProt Synonym Gene Names
C5orf7; JHDM2B; JMJD1B; KIAA1082
UniProt Entry Name
KDM3B_HUMAN
Uniprot Description
JMJD1B: a ubiquitous protein demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Three alternatively spliced isoforms have been reported.
Protein type: Oxidoreductase; EC 1.14.11.-; Demethylase
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm
Molecular Function: dioxygenase activity; metal ion binding
Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification
Research Articles on KDM3B
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Product Notes
The KDM3B kdm3b (Catalog #AAA150057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JMJD1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the KDM3B kdm3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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