Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS1496247 at dilution 1:100)
Rabbit anti-Human MICAL2 Polyclonal Antibody | anti-MICAL2 antibody
MICAL2 Antibody
Gene Names
MICAL2; MICAL-2; MICAL2PV1; MICAL2PV2
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G Purified
Synonyms
MICAL2; Polyclonal Antibody; MICAL2 Antibody; Protein-methionine sulfoxide oxidase MICAL2 (EC:1.14.13.-); KIAA0750; MICAL2PV1; MICAL2PV2; Molecule interacting with CasL protein 2; MICAL-2; anti-MICAL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Form/Format
Liquid
Sequence Length
1124
Applicable Applications for anti-MICAL2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF).
Application Notes
IHC:1:20-1:200, IF:1:50-1:200
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
MICAL2
Immunogen
Recombinant Human [F-actin]-monooxygenase MICAL2 protein (1-495AA)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-135497 / sc-135498
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS1496247 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS1496247 at dilution 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS1496247 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS1496247 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-MICAL2 antibody
Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTFA presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein-methionine sulfoxide oxidase MICAL2 isoform a
NCBI Official Synonym Full Names
microtubule associated monooxygenase, calponin and LIM domain containing 2
NCBI Official Symbol
MICAL2
NCBI Official Synonym Symbols
MICAL-2; MICAL2PV1; MICAL2PV2
NCBI Protein Information
protein-methionine sulfoxide oxidase MICAL2
UniProt Protein Name
Protein-methionine sulfoxide oxidase MICAL2
Protein Family
UniProt Gene Name
MICAL2
UniProt Synonym Gene Names
KIAA0750; MICAL2PV1; MICAL2PV2; MICAL-2
UniProt Entry Name
MICA2_HUMAN
Uniprot Description
MICAL2: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Belongs to the Mical family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.13.-; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 11p15.3
Cellular Component: nucleus
Molecular Function: actin binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; zinc ion binding
Biological Process: actin filament depolymerization; cytoskeleton organization and biogenesis; heart development; heart looping; sulfur oxidation
Research Articles on MICAL2
Similar Products
Product Notes
The MICAL2 mical2 (Catalog #AAA1496247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MICAL2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MICAL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC:1:20-1:200, IF:1:50-1:200. Researchers should empirically determine the suitability of the MICAL2 mical2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MICAL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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