Human Histone Acetyltransferase 1 (HAT1) ELISA Kit | HAT1 elisa kit

Human Histone Acetyltransferase 1 (HAT1) ELISA Kit

Cat. Num. AAA2023707

• Gene Names: HAT1; KAT1
• Reactivity: Human
• Synonyms: Histone Acetyltransferase 1 (HAT1); Human Histone Acetyltransferase 1 (HAT1) ELISA Kit; KAT1; Histone acetyltransferase type B catalytic subunit; HAT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Acetyltransferase 1 (HAT1).; No significant cross-reactivity or interference between Histone Acetyltransferase 1 (HAT1) and analogues was observed.
• Sequence Length: 334

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.103ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Acetyltransferase 1 (HAT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Acetyltransferase 1 (HAT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HAT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Acetyltransferase 1 (HAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Acetyltransferase 1 (HAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Acetyltransferase 1 (HAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Acetyltransferase 1 (HAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HAT1 elisa kit

Signal transduction; Enzyme & Kinase; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 2623156
• NCBI GeneID: 8520
• UniProt Accession #: O14929; Q49A44; Q53QF0; Q53SU4; Q6P594; Q8WWB9
• Molecular Weight: 39,786 Da
• NCBI Official Full Name: histone acetyltransferase 1
• NCBI Official Synonym Full Names: histone acetyltransferase 1
• NCBI Official Symbol: HAT1
• NCBI Official Synonym Symbols: KAT1
• NCBI Protein Information: histone acetyltransferase type B catalytic subunit
• UniProt Protein Name: Histone acetyltransferase type B catalytic subunit
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: HAT1
• UniProt Synonym Gene Names: KAT1
• UniProt Entry Name: HAT1_HUMAN

NCBI Description

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Acetyltransferase; EC 2.3.1.48

Chromosomal Location of Human Ortholog: 2q31.2-q33.1

Cellular Component: nucleoplasm; protein complex; nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; nucleus

Molecular Function: H4 histone acetyltransferase activity; protein binding; histone acetyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation; DNA replication-dependent nucleosome assembly; DNA packaging; response to nutrient; chromatin silencing at telomere