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Western Blot (WB) (All lanes: ATP-dependent RNA helicase DDX39A antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:293T whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 50,37,31 kDaObserved band size: 49 kDa)

Rabbit anti-Human ATP-dependent RNA helicase DDX39A Polyclonal Antibody | anti-DDX39A antibody

Rabbit anti-human ATP-dependent RNA helicase DDX39A polyclonal Antibody(DDX39A)

Gene Names
DDX39A; BAT1; DDXL; BAT1L; DDX39; URH49
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
ATP-dependent RNA helicase DDX39A; Polyclonal Antibody; Rabbit anti-human ATP-dependent RNA helicase DDX39A polyclonal Antibody(DDX39A); DEAD box protein 39; Nuclear RNA helicase URH49; DDX39A; DDX39; anti-DDX39A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
427
Applicable Applications for anti-DDX39A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human ATP-dependent RNA helicase DDX39A protein (178-427aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-68965
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: ATP-dependent RNA helicase DDX39A antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:293T whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 50,37,31 kDaObserved band size: 49 kDa)

Western Blot (WB) (All lanes: ATP-dependent RNA helicase DDX39A antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:293T whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 50,37,31 kDaObserved band size: 49 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin tissue using MBS1496058 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin tissue using MBS1496058 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis using MBS1496058 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS1496058 at dilution 1:100)
Related Product Information for anti-DDX39A antibody
Isoform 1: Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,488 Da
NCBI Official Full Name
ATP-dependent RNA helicase DDX39A
NCBI Official Synonym Full Names
DEAD-box helicase 39A
NCBI Official Symbol
DDX39A
NCBI Official Synonym Symbols
BAT1; DDXL; BAT1L; DDX39; URH49
NCBI Protein Information
ATP-dependent RNA helicase DDX39A
UniProt Protein Name
ATP-dependent RNA helicase DDX39A
UniProt Gene Name
DDX39A
UniProt Synonym Gene Names
DDX39
UniProt Entry Name
DX39A_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

DDX39: Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus. Belongs to the DEAD box helicase family. DECD subfamily.

Protein type: RNA-binding; RNA splicing; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasm; membrane; nucleus; spliceosome

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding

Biological Process: mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; regulation of gene expression; response to DNA damage stimulus; RNA secondary structure unwinding

Research Articles on DDX39A

Similar Products

Product Notes

The DDX39A ddx39a (Catalog #AAA1496058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human ATP-dependent RNA helicase DDX39A polyclonal Antibody(DDX39A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP-dependent RNA helicase DDX39A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the DDX39A ddx39a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP-dependent RNA helicase DDX39A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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