Rabbit anti-Human SGMS2 Polyclonal Antibody | anti-SGMS2 antibody

SGMS2 Antibody

Cat. Num. AAA1494326

• Gene Names: SGMS2; SMS2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G Purified
• Synonyms: SGMS2; Polyclonal Antibody; SGMS2 Antibody; Phosphatidylcholine:ceramide cholinephosphotransferase 2 (EC:2.7.8.27); SMS2; Sphingomyelin synthase 2; anti-SGMS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 365

• Applicable Applications for anti-SGMS2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Phosphatidylcholine:ceramide cholinephosphotransferase 2 protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-34048 / sc-34055 / sc-103883
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat liver tissueAll lanes: SGMS2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43 KDaObserved band size: 43 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS1494326 diluted at 1:600 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS1494326 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-SGMS2 antibody

Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity.

NCBI and Uniprot Product Information

• NCBI GI #: 210147407
• NCBI GeneID: 166929
• NCBI Accession #: NP_001129729.1
• NCBI GenBank Nucleotide #: NM_001136257.1
• UniProt Accession #: Q8NHU3; A8K2S9; B2RA61
• Molecular Weight: 42,280 Da
• NCBI Official Full Name: phosphatidylcholine:ceramide cholinephosphotransferase 2
• NCBI Official Synonym Full Names: sphingomyelin synthase 2
• NCBI Official Symbol: SGMS2
• NCBI Official Synonym Symbols: SMS2
• NCBI Protein Information: phosphatidylcholine:ceramide cholinephosphotransferase 2
• UniProt Protein Name: Phosphatidylcholine:ceramide cholinephosphotransferase 2
• UniProt Gene Name: SGMS2
• UniProt Synonym Gene Names: SMS2
• UniProt Entry Name: SMS2_HUMAN

NCBI Description

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008]

Uniprot Description

SMS2: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. Belongs to the sphingomyelin synthase family.

Protein type: Membrane protein, integral; Transferase; Membrane protein, multi-pass; EC 2.7.8.27; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Golgi apparatus; integral to Golgi membrane; integral to plasma membrane; plasma membrane

Molecular Function: ceramide cholinephosphotransferase activity; kinase activity; sphingomyelin synthase activity

Biological Process: phosphorylation; sphingolipid biosynthetic process; sphingolipid metabolic process; sphingomyelin biosynthetic process